Regulation of cardiac melusin gene expression by hypertrophic stimuli in the rat

J. Aro, H. Tokola, V. P. Ronkainen, E. Koivisto, O. Tenhunen, M. Ilves, I. Szokodi, H. Ruskoaho, J. Rysä

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aim: Melusin is an integrin β1-interacting protein proposed to act as a biomechanical sensor in the heart. We characterized mechanisms and signalling pathways regulating cardiac melusin expression. Methods: Infusion of arginine8-vasopressin (AVP) in Sprague-Dawley (SD) rats, spontaneously hypertensive rats (SHR) and double transgenic rats (dTGR) harbouring both human angiotensinogen and renin genes as well as infusion of angiotensin II (Ang II) in SD rats were used. The effect of direct left ventricular (LV) wall stretch was analysed by using isolated perfused rat heart preparation. For the cell culture studies, mouse atrial HL-1 cell line and neonatal rat ventricular myocytes (NRVMs) were used. Results: Left atrial melusin mRNA levels increased already after 30 min of AVP infusion. Ang II caused significant upregulation of left atrial melusin mRNA (2.1-fold at 6 h, P 

Original languageEnglish
Pages (from-to)470-484
Number of pages15
JournalActa Physiologica
Volume207
Issue number3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Vasopressins
Angiotensin II
Sprague Dawley Rats
Transgenic Rats
Gene Expression
Angiotensinogen
Messenger RNA
Inbred SHR Rats
Renin
Integrins
Muscle Cells
Up-Regulation
Cell Culture Techniques
Cell Line
Genes
Proteins

Keywords

  • Atrial remodelling
  • Extracellular matrix
  • Hypertrophy
  • Melusin

ASJC Scopus subject areas

  • Physiology

Cite this

Aro, J., Tokola, H., Ronkainen, V. P., Koivisto, E., Tenhunen, O., Ilves, M., ... Rysä, J. (2013). Regulation of cardiac melusin gene expression by hypertrophic stimuli in the rat. Acta Physiologica, 207(3), 470-484. https://doi.org/10.1111/apha.12044

Regulation of cardiac melusin gene expression by hypertrophic stimuli in the rat. / Aro, J.; Tokola, H.; Ronkainen, V. P.; Koivisto, E.; Tenhunen, O.; Ilves, M.; Szokodi, I.; Ruskoaho, H.; Rysä, J.

In: Acta Physiologica, Vol. 207, No. 3, 03.2013, p. 470-484.

Research output: Contribution to journalArticle

Aro, J, Tokola, H, Ronkainen, VP, Koivisto, E, Tenhunen, O, Ilves, M, Szokodi, I, Ruskoaho, H & Rysä, J 2013, 'Regulation of cardiac melusin gene expression by hypertrophic stimuli in the rat', Acta Physiologica, vol. 207, no. 3, pp. 470-484. https://doi.org/10.1111/apha.12044
Aro J, Tokola H, Ronkainen VP, Koivisto E, Tenhunen O, Ilves M et al. Regulation of cardiac melusin gene expression by hypertrophic stimuli in the rat. Acta Physiologica. 2013 Mar;207(3):470-484. https://doi.org/10.1111/apha.12044
Aro, J. ; Tokola, H. ; Ronkainen, V. P. ; Koivisto, E. ; Tenhunen, O. ; Ilves, M. ; Szokodi, I. ; Ruskoaho, H. ; Rysä, J. / Regulation of cardiac melusin gene expression by hypertrophic stimuli in the rat. In: Acta Physiologica. 2013 ; Vol. 207, No. 3. pp. 470-484.
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