Regulation of brain prostaglandins by sexual steroids

A. Gecse, A. Ottlecz, G. Telegdy

Research output: Contribution to journalArticle

Abstract

Hypothalamic PGF2(alfa) content was determined by radioimmunoassay in rats. The male animals had significantly higher PGF2(alpha) level than the females. The hypothalamic PGF2(alpha) content increased following orchidectomy (GDX), while ovariectomy (OVX) had no such effect. Administration of progesterone (100 μg/kg or 1 mg/kg i.m.) alone or combined with oestrone (100 μg/kg or 1 mg/kg i.m.) resulted in a significant decrease of PGF2(alpha) level in the hypothalamus, while oestrone (100 μg/kg or 1 mg/kg i.m.) alone was ineffective. Testosterone (1 or 5 mg/kg i.m.) in GDX animals restored the hypothalamic PGF2(alpha) content to the control level. The synthesis and breakdown of prostaglandins were also studied in the microsomal and cytosol fraction of brain homogenate. The PGD2 was the main product of the arachidonate cascade in rat brain microsomes. OVX increased the formation of PGD2 and diminished the biosynthesis of PGF2(alpha) Oestrone administration to OVX rats enhanced the formation of PGF2(alpha) Progesterone injection decreased the biosynthesis of PGF2(alpha) and PGD2 in OVX animals. Combined administration of progesterone and oestrone to OVX rats failed to restore the PGF2(alpha) synthesis. In orchidectomized animals, the arachidonate cascade was found to be depressed; further the synthesis of prostaglandins was normalized after testosterone substitution. The inactivation of 3H-PGF2(alpha) by rat brain cytosol fraction was negligible.

Original languageEnglish
Pages (from-to)155-161
Number of pages7
JournalActa Physiologica Academiae Scientiarum Hungaricae
Volume57
Issue number2
Publication statusPublished - 1981

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Dinoprost
Prostaglandins
Steroids
Brain
Estrone
Prostaglandin D2
Progesterone
Cytosol
Orchiectomy
Ovariectomy
Microsomes
Hypothalamus
Radioimmunoassay
Testosterone
Injections

ASJC Scopus subject areas

  • Physiology

Cite this

Regulation of brain prostaglandins by sexual steroids. / Gecse, A.; Ottlecz, A.; Telegdy, G.

In: Acta Physiologica Academiae Scientiarum Hungaricae, Vol. 57, No. 2, 1981, p. 155-161.

Research output: Contribution to journalArticle

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