Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk

Farhad Abtahian, Anastasia Guerriero, Eric Sebzda, Min Min Lu, Rong Zhou, Attila Mocsai, Erin E. Myers, Bin Huang, David G. Jackson, Victor A. Ferrari, Victor Tybulewicz, Clifford A. Lowell, John J. Lepore, Gary A. Koretzky, Mark L. Kahn

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294 Citations (Scopus)

Abstract

Lymphatic vessels develop from specialized endothelial cells in preexisting blood vessels, but the molecular signals that regulate this separation are unknown. Here we identify a failure to separate emerging lymphatic vessels from blood vessels in mice lacking the hematopoietic signaling protein SLP-76 or Syk. Blood-lymphatic connections lead to embryonic hemorrhage and arteriovenous shunting. Expression of sip-76 could not be detected in endothelial cells, and blood-filled lymphatics also arose in wild-type mice reconstituted with SLP-76 - deficient bone marrow. These studies reveal a hematopoietic signaling pathway required for separation of the two major vascular networks in mammals.

Original languageEnglish
Pages (from-to)247-251
Number of pages5
JournalScience
Volume299
Issue number5604
DOIs
Publication statusPublished - Jan 10 2003

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Cite this

Abtahian, F., Guerriero, A., Sebzda, E., Lu, M. M., Zhou, R., Mocsai, A., Myers, E. E., Huang, B., Jackson, D. G., Ferrari, V. A., Tybulewicz, V., Lowell, C. A., Lepore, J. J., Koretzky, G. A., & Kahn, M. L. (2003). Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk. Science, 299(5604), 247-251. https://doi.org/10.1126/science.1079477