Regulated appearance of NMDA receptor subunits and channel functions during in vitro neuronal differentiation

Márta Jelitai, K. Schlett, Patrícia Varju, Ulrich Eisel, E. Madarász

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The schedule of NMDA receptor subunit expression and the appearance of functional NMDA-gated ion channels were investigated during the retinoic acid (RA) induced neuronal differentiation of NE-4C, a p53-deficient mouse neuroectodermal progenitor cell line. NR2A, NR2B, and NR2D subunit transcripts were present in both nondifferentiated and neuronally differentiated cultures, while NR2C subunits were expressed only transiently, during the early period of neural differentiation. Several splice variants of NR1 were detected in noninduced progenitors and in RA-induced cells, except the N1 exon containing transcripts that appeared after the fourth day of induction, when neuronal processes were already formed. NR1 and NR2A subunit proteins were detected both in nondifferentiated progenitor cells and in neurons, while the mature form of NR2B subunit protein appeared only at the time of neuronal process elongation. Despite the early presence of NR1 and NR2A subunits, NMDA-evoked responses could be detected in NE-4C neurons only after the sixth day of induction, coinciding in time with the expression of the mature NR2B subunit. The formation of functional NMDA receptors also coincided with the appearance of synapsin I and synaptophysin. The lag period between the production of the subunits and the onset of channel function suggests that subunits capable of channel formation cannot form functional NMDA receptors until a certain stage of neuronal commitment. Thus, the in vitro neurogenesis by NE-4C cells provides a suitable tool to investigate some inherent regulatory processes involved in the initial maturation of NMDA receptor complexes.

Original languageEnglish
Pages (from-to)54-65
Number of pages12
JournalJournal of Neurobiology
Volume51
Issue number1
DOIs
Publication statusPublished - 2002

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N-Methyl-D-Aspartate Receptors
Protein Subunits
N-Methylaspartate
Tretinoin
Stem Cells
Synapsins
Neurons
Synaptophysin
Neurogenesis
Ion Channels
Exons
Appointments and Schedules
Cell Line
In Vitro Techniques

Keywords

  • Cell line
  • Neuronal development
  • NR1 splice variants
  • NR2 subunits
  • Progenitor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regulated appearance of NMDA receptor subunits and channel functions during in vitro neuronal differentiation. / Jelitai, Márta; Schlett, K.; Varju, Patrícia; Eisel, Ulrich; Madarász, E.

In: Journal of Neurobiology, Vol. 51, No. 1, 2002, p. 54-65.

Research output: Contribution to journalArticle

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