Regular transcutaneous myostimulation alters skeletal muscle phospholipid fatty acid composition and oxidative stability in rabbits

András Szabó, H. Fébel, M. Mézes, Z. Szendrő, S. Miklós, R. Romvári

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This experiment was performed to study the effects of regular, isometric, electrically induced contractions on skeletal muscle phospholipid (PL) and triglyceride (TG) fatty acid profile and oxidative stability. Transcutaneous electrical myostimulation of longissimus dorsi (30 Hz, 20 ms impulse length, 10 mA, for 2×20 min daily) was performed on 8 Pannon White male rabbits for 4 weeks; 8 animals were set as control. Muscle TG and PL fatty acid profiles were determined by capillary gas chromatography after lipid fractionation. In the PL, the proportion of pentadecanoic (C15:0), palmitic (C16:0), linoleic (C18:2 n-6) and γ-linolenic (C18:3 n-6) acids decreased significantly compared to the control, as well as the sum of total saturated fatty acids. Increasing proportions of palmitoleic (C16:1 n-7), gondoic (C20:1 n-9), arachidonic (C20:4 n-6), eicosapentaenoic (C20:5 n-3) and docosahexaenoic (C22:6 n-3) acids were measured. The C20:4 n-6/C20:3 n-6 ratio (estimated Δ5 desaturase activity), the sum of polyunsaturated fatty acids (PUFA) of 20 to 22 carbon chain length, the sum of total n-6 fatty acids and the unsaturation index increased. In the TG, stearic acid (C18:0) proportion was significantly decreased by the stimulation. Electrical treatment increased oxidative stability as assessed by thiobarbituric acid reactive substances (TBARS).

Original languageEnglish
Pages (from-to)193-202
Number of pages10
JournalActa Physiologica Hungarica
Volume92
Issue number3-4
DOIs
Publication statusPublished - 2005

Fingerprint

Phospholipids
Skeletal Muscle
Fatty Acids
Rabbits
Triglycerides
Acids
Thiobarbituric Acid Reactive Substances
Unsaturated Fatty Acids
Gas Chromatography
Carbon
Lipids
Muscles

Keywords

  • Phospholipid
  • Rabbit
  • Skeletal muscle
  • TBARS
  • Transcutaneous electrical myostimulation
  • Triglyceride

ASJC Scopus subject areas

  • Physiology

Cite this

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title = "Regular transcutaneous myostimulation alters skeletal muscle phospholipid fatty acid composition and oxidative stability in rabbits",
abstract = "This experiment was performed to study the effects of regular, isometric, electrically induced contractions on skeletal muscle phospholipid (PL) and triglyceride (TG) fatty acid profile and oxidative stability. Transcutaneous electrical myostimulation of longissimus dorsi (30 Hz, 20 ms impulse length, 10 mA, for 2×20 min daily) was performed on 8 Pannon White male rabbits for 4 weeks; 8 animals were set as control. Muscle TG and PL fatty acid profiles were determined by capillary gas chromatography after lipid fractionation. In the PL, the proportion of pentadecanoic (C15:0), palmitic (C16:0), linoleic (C18:2 n-6) and γ-linolenic (C18:3 n-6) acids decreased significantly compared to the control, as well as the sum of total saturated fatty acids. Increasing proportions of palmitoleic (C16:1 n-7), gondoic (C20:1 n-9), arachidonic (C20:4 n-6), eicosapentaenoic (C20:5 n-3) and docosahexaenoic (C22:6 n-3) acids were measured. The C20:4 n-6/C20:3 n-6 ratio (estimated Δ5 desaturase activity), the sum of polyunsaturated fatty acids (PUFA) of 20 to 22 carbon chain length, the sum of total n-6 fatty acids and the unsaturation index increased. In the TG, stearic acid (C18:0) proportion was significantly decreased by the stimulation. Electrical treatment increased oxidative stability as assessed by thiobarbituric acid reactive substances (TBARS).",
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T1 - Regular transcutaneous myostimulation alters skeletal muscle phospholipid fatty acid composition and oxidative stability in rabbits

AU - Szabó, András

AU - Fébel, H.

AU - Mézes, M.

AU - Szendrő, Z.

AU - Miklós, S.

AU - Romvári, R.

PY - 2005

Y1 - 2005

N2 - This experiment was performed to study the effects of regular, isometric, electrically induced contractions on skeletal muscle phospholipid (PL) and triglyceride (TG) fatty acid profile and oxidative stability. Transcutaneous electrical myostimulation of longissimus dorsi (30 Hz, 20 ms impulse length, 10 mA, for 2×20 min daily) was performed on 8 Pannon White male rabbits for 4 weeks; 8 animals were set as control. Muscle TG and PL fatty acid profiles were determined by capillary gas chromatography after lipid fractionation. In the PL, the proportion of pentadecanoic (C15:0), palmitic (C16:0), linoleic (C18:2 n-6) and γ-linolenic (C18:3 n-6) acids decreased significantly compared to the control, as well as the sum of total saturated fatty acids. Increasing proportions of palmitoleic (C16:1 n-7), gondoic (C20:1 n-9), arachidonic (C20:4 n-6), eicosapentaenoic (C20:5 n-3) and docosahexaenoic (C22:6 n-3) acids were measured. The C20:4 n-6/C20:3 n-6 ratio (estimated Δ5 desaturase activity), the sum of polyunsaturated fatty acids (PUFA) of 20 to 22 carbon chain length, the sum of total n-6 fatty acids and the unsaturation index increased. In the TG, stearic acid (C18:0) proportion was significantly decreased by the stimulation. Electrical treatment increased oxidative stability as assessed by thiobarbituric acid reactive substances (TBARS).

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KW - Phospholipid

KW - Rabbit

KW - Skeletal muscle

KW - TBARS

KW - Transcutaneous electrical myostimulation

KW - Triglyceride

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JF - Physiology International

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