Regular exercise reduces 8-oxodG in the nuclear and mitochondrial DNA and modulates the DNA repair activity in the liver of old rats

Hideko Nakamoto, Takao Kaneko, Shoichi Tahara, Eri Hayashi, Hisashi Naito, Z. Radák, S. Goto

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Exercise is often said to increase the generation of reactive oxygen species that are potentially harmful. On the other hand, regular exercise has various health benefits even late in life. The specific aim of this study was to explore effects of regular exercise on oxidative status of DNA in aged animals. We report that 2 months of regular treadmill running of aged rats (21 month old) significantly reduced 8-oxodG content to the level of young adult animals (11 month old) in both nuclear and mitochondrial DNA of the liver. The mitochondrial DNA showed 10-fold higher content of the oxidative lesion than the nuclear DNA. The levels in old animals were 2- and 1.5-fold higher than that in young adults for the nucleus and mitochondria, respectively. The activity of the repair enzyme OGG1 was upregulated significantly in the nucleus but not in mitochondria by the exercise. To our knowledge, this is the first report demonstrating that regular exercise can reduce significantly oxidative damage to both the nuclear and mitochondrial DNA. We suggest that the apparent beneficial outcomes in reducing the DNA damage by regular exercise can be interpreted in terms of hormetic effect by moderate oxidative stress and potential adaptation to stronger stresses.

Original languageEnglish
Pages (from-to)287-295
Number of pages9
JournalExperimental Gerontology
Volume42
Issue number4
DOIs
Publication statusPublished - Apr 2007

Fingerprint

Mitochondrial DNA
DNA Repair
Liver
Rats
Animals
Mitochondria
Repair
Exercise
DNA
Exercise equipment
Oxidative stress
Young Adult
Reactive Oxygen Species
Health
Insurance Benefits
Enzymes
Running
DNA Damage
8-oxo-7-hydrodeoxyguanosine
Oxidative Stress

Keywords

  • 8-oxodG
  • Aging
  • DNA oxidation
  • DNA repair
  • Liver
  • Mitochondria
  • Nucleus
  • OGG1
  • Regular exercise

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

Cite this

Regular exercise reduces 8-oxodG in the nuclear and mitochondrial DNA and modulates the DNA repair activity in the liver of old rats. / Nakamoto, Hideko; Kaneko, Takao; Tahara, Shoichi; Hayashi, Eri; Naito, Hisashi; Radák, Z.; Goto, S.

In: Experimental Gerontology, Vol. 42, No. 4, 04.2007, p. 287-295.

Research output: Contribution to journalArticle

Nakamoto, Hideko ; Kaneko, Takao ; Tahara, Shoichi ; Hayashi, Eri ; Naito, Hisashi ; Radák, Z. ; Goto, S. / Regular exercise reduces 8-oxodG in the nuclear and mitochondrial DNA and modulates the DNA repair activity in the liver of old rats. In: Experimental Gerontology. 2007 ; Vol. 42, No. 4. pp. 287-295.
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