Regular exercise enhances blood pressure lowering effect of acetylcholine by increased contribution of nitric oxide

Gabriella Dörnyei, E. Monos, G. Kaley, Á Koller

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This study is aimed to test the hypothesis, that short-term daily bouts of exercise alter the endothelial regulation of peripheral vascular resistance by nitric oxide. Rats ran on a treadmill once a day, 5 days a week, for an average of three weeks with gradually increasing intensity (EX), while a control group remained sedentary (SED). Dose dependent reductions in mean arterial blood pressure (resting MABP; SED: 120.0±3.4 and EX: 127.8±4.0 mm Hg) of pentobarbital anesthetized rats to intravenous endothelium independent dilator sodium nitroprusside (SNP; 0.6-3.0 μg/kg) were not different in EX and SED animals. In contrast, dose dependent reductions in MABP to endothelium dependent dilator acetylcholine (ACh) were significantly enhanced in EX compared to those in SED rats (at 0.5 and 1.0 μg/kg ACh: 60.3±2.4 and 66.5±1.8 vs 52.8±2.0 and 59.8±1.7 mmHg, respectively, pω-nitro-L-arginine (L-NNA, a nitric oxide synthase inhibitor) elicited a similar increase (∼30%) in the MABP in the two groups and eliminated the difference between ACh-induced blood pressure lowering responses in EX and SED rats (at 0.5 and 1.0 μg/kg ACh: 44.6±4.7 and 56.3±4.4 vs 50.9±4.5 and 59.4±3.6 mm Hg, respectively). Thus, we suggest that the enhanced acetylcholine-induced decrease in systemic blood pressure following regular daily exercise is primarily due to the augmented synthesis of nitric oxide in the endothelium of peripheral vasculature. This change in the function of endothelium could be important in the adaptation of circulation to exercise training.

Original languageEnglish
Pages (from-to)127-138
Number of pages12
JournalActa Physiologica Hungarica
Volume87
Issue number2
Publication statusPublished - 2000

Fingerprint

Acetylcholine
Nitric Oxide
Endothelium
Blood Pressure
Vascular Resistance
Arterial Pressure
Nitroprusside
Pentobarbital
Nitric Oxide Synthase
Single Nucleotide Polymorphism
Arginine
Exercise
Control Groups

Keywords

  • Exercise
  • L-NNA
  • Nitric oxide
  • Peripheral resistance
  • Rats
  • Sodium nitroprusside

ASJC Scopus subject areas

  • Physiology

Cite this

Regular exercise enhances blood pressure lowering effect of acetylcholine by increased contribution of nitric oxide. / Dörnyei, Gabriella; Monos, E.; Kaley, G.; Koller, Á.

In: Acta Physiologica Hungarica, Vol. 87, No. 2, 2000, p. 127-138.

Research output: Contribution to journalArticle

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abstract = "This study is aimed to test the hypothesis, that short-term daily bouts of exercise alter the endothelial regulation of peripheral vascular resistance by nitric oxide. Rats ran on a treadmill once a day, 5 days a week, for an average of three weeks with gradually increasing intensity (EX), while a control group remained sedentary (SED). Dose dependent reductions in mean arterial blood pressure (resting MABP; SED: 120.0±3.4 and EX: 127.8±4.0 mm Hg) of pentobarbital anesthetized rats to intravenous endothelium independent dilator sodium nitroprusside (SNP; 0.6-3.0 μg/kg) were not different in EX and SED animals. In contrast, dose dependent reductions in MABP to endothelium dependent dilator acetylcholine (ACh) were significantly enhanced in EX compared to those in SED rats (at 0.5 and 1.0 μg/kg ACh: 60.3±2.4 and 66.5±1.8 vs 52.8±2.0 and 59.8±1.7 mmHg, respectively, pω-nitro-L-arginine (L-NNA, a nitric oxide synthase inhibitor) elicited a similar increase (∼30{\%}) in the MABP in the two groups and eliminated the difference between ACh-induced blood pressure lowering responses in EX and SED rats (at 0.5 and 1.0 μg/kg ACh: 44.6±4.7 and 56.3±4.4 vs 50.9±4.5 and 59.4±3.6 mm Hg, respectively). Thus, we suggest that the enhanced acetylcholine-induced decrease in systemic blood pressure following regular daily exercise is primarily due to the augmented synthesis of nitric oxide in the endothelium of peripheral vasculature. This change in the function of endothelium could be important in the adaptation of circulation to exercise training.",
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