Regional expression of the type 3 iodothyronine deiodinase messenger ribonucleic acid in the rat central nervous system and its regulation by thyroid hormone

Helen M. Tu, Gabor Legradi, T. Bartha, Domenico Salvatore, Ronald M. Lechan, P. Reed Larsen

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Type 3 iodothyronine deiodinase (D3) is a selenoenzyme that inactivates thyroid hormone. It is necessary for T3 homeostasis in the central nervous system. D3 activity has been identified in many regions of the brain and parallels thyroid status, but the level at which it is regulated and its specific cellular locations are not known. We evaluated the effect of thyroid status on the expression of the D3 gene within the central nervous system using in situ hybridization histochemistry. D3 messenger RNA (mRNA) was identified throughout, but with high focal expression in the hippocampal pyramidal neurons, granule cells of the dentate nucleus, and layers II-VI of the cerebral cortex. In every region, D3 mRNA abundance was correlated with thyroid status. Four different D3 transcripts were identified by Northern analyses, with evidence for region-specific processing, and D3 mRNA increased 4- to 50-fold from the euthyroid to the hyperthyroid state. D3 mRNA was not detectable in hypothyroid brain. In the central nervous system, the D3 gene is highly T3 responsive, and its focal localization within the hippocampus and cerebral cortex suggests an important role for T3 homeostasis in memory and cognitive functions.

Original languageEnglish
Pages (from-to)784-790
Number of pages7
JournalEndocrinology
Volume140
Issue number2
DOIs
Publication statusPublished - 1999

Fingerprint

Iodide Peroxidase
Thyroid Hormones
Central Nervous System
RNA
Messenger RNA
Thyroid Gland
Cerebral Cortex
Homeostasis
Cerebellar Nuclei
Pyramidal Cells
Brain
Hyperthyroidism
Cell Nucleus
Cognition
In Situ Hybridization
Hippocampus
Gene Expression
3-monoiodothyronine
Genes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Regional expression of the type 3 iodothyronine deiodinase messenger ribonucleic acid in the rat central nervous system and its regulation by thyroid hormone. / Tu, Helen M.; Legradi, Gabor; Bartha, T.; Salvatore, Domenico; Lechan, Ronald M.; Larsen, P. Reed.

In: Endocrinology, Vol. 140, No. 2, 1999, p. 784-790.

Research output: Contribution to journalArticle

Tu, Helen M. ; Legradi, Gabor ; Bartha, T. ; Salvatore, Domenico ; Lechan, Ronald M. ; Larsen, P. Reed. / Regional expression of the type 3 iodothyronine deiodinase messenger ribonucleic acid in the rat central nervous system and its regulation by thyroid hormone. In: Endocrinology. 1999 ; Vol. 140, No. 2. pp. 784-790.
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