Regional distribution of type 2 thyroxine deiodinase messenger ribonucleic acid in rat hypothalamus and pituitary and its regulation by thyroid hormone

Helen M. Tu, Sung Woo Kim, Domenico Salvatore, T. Bartha, Gabor Legradi, P. Reed Larsen, Ronald M. Lechan

Research output: Contribution to journalArticle

224 Citations (Scopus)

Abstract

To identify the specific locations of type 2 deiodinase (D2) messenger RNA (mRNA) in the hypothalamus and pituitary gland and determine its regulation by thyroid hormone, we performed in situ hybridization histochemistry, Northern analysis, and quantitative RT-PCR in euthyroid, hypothyroid, and hyperthyroid rats. By in situ hybridization histochemistry, silver grains were concentrated over ependymal cells lining the floor and infralateral walls of the third ventricle extending from the rostral tip of the median eminence (ME) to the infundibular recess, surrounding blood vessels in the arcuate nucleus (ARC), and in the ME adjacent to the portal vessels and overlying the tuberoinfundibular sulci. Silver grams also accumulated over distinct cells in the midportion of the anterior pituitary. In hypothyroid animals, an increase in signal intensity was observed in the caudal hypothalamus, and a marked increase in the number of positive cells occurred in the anterior pituitary. Microdissection of the hypothalamus for Northern and PCR analysis established the authenticity of D2 mRNA in the caudal hypothalamus, and confirmed that the majority of D2 mRNA is concentrated in this region. The distribution of D2 mRNA suggests its expression in specialized ependymal cells, termed tanycytes, originating from the third ventricle. Thus, the tanycyte is the source of the high D2 activity previously found in the ARC-ME region of the hypothalamus. The results indicate that tanycytes may have a previously unrecognized integral role in feedback regulation of TSH secretion by T4.

Original languageEnglish
Pages (from-to)3359-3368
Number of pages10
JournalEndocrinology
Volume138
Issue number8
DOIs
Publication statusPublished - 1997

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Iodide Peroxidase
Thyroxine
Thyroid Hormones
Hypothalamus
Median Eminence
RNA
Ependymoglial Cells
Messenger RNA
Arcuate Nucleus of Hypothalamus
Third Ventricle
Silver
In Situ Hybridization
Polymerase Chain Reaction
Microdissection
Pituitary Gland
Hyperthyroidism
Blood Vessels
Cell Count

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Regional distribution of type 2 thyroxine deiodinase messenger ribonucleic acid in rat hypothalamus and pituitary and its regulation by thyroid hormone. / Tu, Helen M.; Kim, Sung Woo; Salvatore, Domenico; Bartha, T.; Legradi, Gabor; Larsen, P. Reed; Lechan, Ronald M.

In: Endocrinology, Vol. 138, No. 8, 1997, p. 3359-3368.

Research output: Contribution to journalArticle

Tu, Helen M. ; Kim, Sung Woo ; Salvatore, Domenico ; Bartha, T. ; Legradi, Gabor ; Larsen, P. Reed ; Lechan, Ronald M. / Regional distribution of type 2 thyroxine deiodinase messenger ribonucleic acid in rat hypothalamus and pituitary and its regulation by thyroid hormone. In: Endocrinology. 1997 ; Vol. 138, No. 8. pp. 3359-3368.
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