Regional differences in the neuronal expression of cyclooxygenase-2 (COX-2) in the newborn pig brain

Orsolya Oláh, István Németh, Valéria Tóth-Szuki, Ferenc Bari, Ferenc Domoki

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3 Citations (Scopus)

Abstract

Cycoxygene (COX-2 is the major costitutively expressed COX isoform in the newborn brain. COX-2 derived prostanoids and reactive oxygen species appear to play a major role in the mechanism of perinatal hypoxic-ischemic injury in the newborn piglet, an accepted animal model of the human term neonate. The study aimed to quantitatively determine COX-2 immunopositive neurons in different brain regions in piglets under normoxic conditions (n=15), and 4 hours after 10 min asphyxia (n=11). Asphyxia did not induce significant changes in neuronal COX-2 expression of any studied brain areas. In contrast, there was a marked regional difference in all experimental groups. Thus, significant difference was observed between fronto-parietal and temporo-occipital regions: 59±4% and 67±3% versus 41±2%* and 31±3%* respectively (mean±SEM, data are pooled from all subjects, n=26, *p<0.05, vs. fronto-parietal region). In the hippocampus, COX-2 immunopositivity was rare (highest expression in CA1 region: 14±2%). The studied subcortical areas showed negligible COX-2 staining. Our findings suggest that asphyxia does not significantly alter the pattern of neuronal COX-2 expression in the early reventilation period. Furthermore, based on the striking differences observed in cortical neuronal COX-2 distribution, the contribution of COX-2 mediated neuronal injury after asphyxia may also show region-specific differences.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalActa Histochemica et Cytochemica
Volume45
Issue number3
DOIs
Publication statusPublished - Jul 10 2012

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Keywords

  • Cerebral cortex
  • Hippocampus
  • Immuno-histochemistry
  • Neonatal pig
  • Prostaglandin H synthase

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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