Regional brain activation by bicuculline visualized by functional magnetic resonance imaging. Time-resolved assessment of bicuculline-induced changes in local cerebral blood volume using an intravascular contrast agent

Torsten Reese, Börje Bjelke, Robert Porszasz, Diana Baumann, Damien Bochelen, André Sauter, Markus Rudin

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Functional magnetic resonance imaging (fMRI) has been applied to study rat focal brain activation induced by intravenous administration of the GABA(A) antagonist bicuculline. Using magnetite nanoparticles as a blood pool contrast agent, local changes in cerebral blood volume (CBV) were assessed with high temporal (10 s) and spatial (0.35 x 0.6 mm2) resolutions. Upon infusion of the bicuculline region-specific increases in CBV have been observed, suggesting CBV to reflect brain activity. During the first 2 min, the signal increases were predominant in the cortex, followed by increases in other brain areas, such as the caudate putamen, thalamus and cerebellum. Ten minutes after the start of infusion, a dominant response was observed in the thalamus, while in the caudate putamen a biphasic response pattern was seen. The magnitude of the signal responses in all brain regions was dependent on the dose of bicuculline and, in general, matched the known distribution of GABA(A) binding sites. This study suggests that pharmacological fMRI, displaying brain function at the highly specific level of drug-receptor interaction, should foster our understanding of normal and pathological brain function.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalNMR in biomedicine
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 2000

Keywords

  • Bicuculline
  • Cerebral blood volume
  • Functional MRI (fMRI)
  • Intravascular contrast agent
  • Pharmacological stimulation

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

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