Regeneration of reinnervated rat soleus muscle is accompanied by fiber transition toward a faster phenotype

Luca Mendler, S. Pintér, Monika Kiricsi, Zsuzsanna Baka, L. Dux

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The functional recovery of skeletal muscles after peripheral nerve transection and microsurgical repair is generally incomplete. Several reinnervation abnormalities have been described even after nerve reconstruction surgery. Less is known, however, about the regenerative capacity of reinnervated muscles. Previously, we detected remarkable morphological and motor endplate alterations after inducing muscle necrosis and subsequent regeneration in the reinnervated rat soleus muscle. In the present study, we comparatively analyzed the morphometric properties of different fiber populations, as well as the expression pattern of myosin heavy chain isoforms at both immunohistochemical and mRNA levels in reinnervated versus reinnervated-regenerated muscles. A dramatic slow-to-fast fiber type transition was found in reinnervated soleus, and a further change toward the fast phenotype was observed in reinnervated-regenerated muscles. These findings suggest that the (fast) pattern of reinnervation plays a dominant role in the specification of fiber phenotype during regeneration, which can contribute to the long-lasting functional impairment of the reinnervated muscle. Moreover, because the fast II fibers (and selectively, a certain population of the fast IIB fibers) showed better recovery than did the slow type I fibers, the faster phenotype of the reinnervated-regenerated muscle seems to be actively maintained by selective yet undefined cues.

Original languageEnglish
Pages (from-to)111-123
Number of pages13
JournalJournal of Histochemistry and Cytochemistry
Volume56
Issue number2
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Regeneration
Skeletal Muscle
Phenotype
Muscles
Motor Endplate
Myosin Heavy Chains
Peripheral Nerves
Population
Cues
Protein Isoforms
Necrosis
Messenger RNA

Keywords

  • Fiber type transition
  • Myosin heavy chain
  • Notexin
  • Rat
  • Regeneration
  • Reinnervation
  • Soleus

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Regeneration of reinnervated rat soleus muscle is accompanied by fiber transition toward a faster phenotype. / Mendler, Luca; Pintér, S.; Kiricsi, Monika; Baka, Zsuzsanna; Dux, L.

In: Journal of Histochemistry and Cytochemistry, Vol. 56, No. 2, 02.2008, p. 111-123.

Research output: Contribution to journalArticle

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