Regeneration of isolated mesophyll and cell suspension protoplasts to plants in Stylosanthes guianensis. A tropical forage legume

L. Szabados, W. M. Roca

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Protoplasts were isolated from leaf mesophyll and cell suspensions of two accessions of Stylosanthes guianensis (Aubl.), a tropical forage legume. When cultured in VKM liquid culture medium, both types of protoplasts divided at a rate of 4-8%, and subsequently formed cell colonies. Protoplast-derived calluses produced numerous shoots when transferred to regeneration medium. Regenerated shoots could be easily rooted, and plantlets were transferred to soil. The effects of several factors on the efficiency of this protoplast system have been investigated.

Original languageEnglish
Pages (from-to)174-177
Number of pages4
JournalPlant Cell Reports
Volume5
Issue number3
DOIs
Publication statusPublished - Jun 1 1986

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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