Regeneration of haploid plants after distant pollination of wheat via zygote rescue

Ferenc Bakos, Katalin Jäger, B. Barnabás

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Haploid wheat plants have been produced by a new method of zygote rescue carried out after distant pollination. Wheat stigmas were pollinated with maize pollen and subsequently the activated egg cells from the elongated ovaries were rescued for in vitro plant development in single cell culture. As the control, 2-week-old embryos were also dissected and then cultured. The efficiency of both techniques was comparable. Wheat was also pollinated with rice, and the further development of rescued zygotes into multicellular structures is reported here for the first time. Because the lack of a normal endosperm hampers embryo development even in the early stages, early zygote rescue (two days after distant pollination) may represent a more efficient way of producing double haploid (DH) plants in cultivars that are recalcitrant in androgenic cultures, after further optimization of in vitro culture of isolated single cells.

Original languageEnglish
Pages (from-to)167-171
Number of pages5
JournalActa Biologica Cracoviensia Series Botanica
Volume47
Issue number1
Publication statusPublished - 2005

Fingerprint

zygote
haploidy
pollination
wheat
ova
stigma
in vitro culture
embryo (plant)
endosperm
plant development
cell culture
embryogenesis
pollen
rice
corn
cultivars
methodology
cells

Keywords

  • Activated egg cell
  • Co-culture
  • Double haploid
  • Embryo rescue
  • Haploid
  • Nurse culture
  • Parthenogenesis
  • Wheat x maize
  • Zygote rescue

ASJC Scopus subject areas

  • Plant Science

Cite this

Regeneration of haploid plants after distant pollination of wheat via zygote rescue. / Bakos, Ferenc; Jäger, Katalin; Barnabás, B.

In: Acta Biologica Cracoviensia Series Botanica, Vol. 47, No. 1, 2005, p. 167-171.

Research output: Contribution to journalArticle

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