Regeneration of fertile wheat (Triticum aestivum) plants from isolated zygotes using wheat microspore culture as nurse cells

F. Bakos, É Darkó, Zs Pónya, B. Barnabás

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Abstract

A new method for growing isolated wheat zygotes by co-cultivation with microspores isolated from the same species has been developed. Although the mortality of isolated zygotes within 6 h after the transfer into the nurse culture was relatively high (70%) in this method, the majority of the structures which survived developed into fertile plants (61%). Zygotic structures grown in these cultures were morphologically not as regular as embryos grown in planta, but they resembled them closely in cytological properties and developmental pattern.

Original languageEnglish
Pages (from-to)243-247
Number of pages5
JournalPlant Cell, Tissue and Organ Culture
Volume74
Issue number3
DOIs
Publication statusPublished - Sep 1 2003

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Keywords

  • Co-cultivation
  • Embryo
  • Hormone-like compounds
  • in vitro
  • in vivo fertilized zygotes

ASJC Scopus subject areas

  • Horticulture

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