Fertilis dihaploid növények eloállítása egysejtmagvas mikrospórák korai kolhicin kezelésével kukorica-(Zea mays L.) antérakultúrában

Translated title of the contribution: Regeneration of fertile doubled haploid plants from colhicine-supplemented medium in maize (Zea mays L.) anther culture

G. Kovács, Bohus Obert, B. Barnabás

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Genome doubling is critical for obtaining fertile doubled haploid plants from maize anther culture. The most common chromosome doubling method applies colchicine to the plants. However, the conventional treatment has several disadvantages. In this experiment, anther cultures of one maize F1 hybrid were treated with 0.02% and 0.03% colchicine for three days at the beginning of microspore induction. Colchicine added to the induction medium had no negative effect on the androgenic response of the genotype examined. The spontaneous doubling rate of the hybrid was very low, underlying the need for an efficient genome doubling system. Significantly higher fertility was observed in plants originating from the colchicine-supplemented induction medium. In conclusion, the results obtained clearly demonstrate that the application of colchicine prior to the first microspore mitosis was superior to the conventionally used chromosome doubling techniques.

Original languageHungarian
Pages (from-to)13-23
Number of pages11
JournalNovenytermeles
Volume48
Issue number1
Publication statusPublished - Feb 1999

Fingerprint

anther culture
colchicine
doubled haploids
Zea mays
corn
microspores
chromosomes
genome
mitosis
genotype
methodology

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "Fertilis dihaploid n{\"o}v{\'e}nyek elo{\'a}ll{\'i}t{\'a}sa egysejtmagvas mikrosp{\'o}r{\'a}k korai kolhicin kezel{\'e}s{\'e}vel kukorica-(Zea mays L.) ant{\'e}rakult{\'u}r{\'a}ban",
abstract = "Genome doubling is critical for obtaining fertile doubled haploid plants from maize anther culture. The most common chromosome doubling method applies colchicine to the plants. However, the conventional treatment has several disadvantages. In this experiment, anther cultures of one maize F1 hybrid were treated with 0.02{\%} and 0.03{\%} colchicine for three days at the beginning of microspore induction. Colchicine added to the induction medium had no negative effect on the androgenic response of the genotype examined. The spontaneous doubling rate of the hybrid was very low, underlying the need for an efficient genome doubling system. Significantly higher fertility was observed in plants originating from the colchicine-supplemented induction medium. In conclusion, the results obtained clearly demonstrate that the application of colchicine prior to the first microspore mitosis was superior to the conventionally used chromosome doubling techniques.",
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T1 - Fertilis dihaploid növények eloállítása egysejtmagvas mikrospórák korai kolhicin kezelésével kukorica-(Zea mays L.) antérakultúrában

AU - Kovács, G.

AU - Obert, Bohus

AU - Barnabás, B.

PY - 1999/2

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N2 - Genome doubling is critical for obtaining fertile doubled haploid plants from maize anther culture. The most common chromosome doubling method applies colchicine to the plants. However, the conventional treatment has several disadvantages. In this experiment, anther cultures of one maize F1 hybrid were treated with 0.02% and 0.03% colchicine for three days at the beginning of microspore induction. Colchicine added to the induction medium had no negative effect on the androgenic response of the genotype examined. The spontaneous doubling rate of the hybrid was very low, underlying the need for an efficient genome doubling system. Significantly higher fertility was observed in plants originating from the colchicine-supplemented induction medium. In conclusion, the results obtained clearly demonstrate that the application of colchicine prior to the first microspore mitosis was superior to the conventionally used chromosome doubling techniques.

AB - Genome doubling is critical for obtaining fertile doubled haploid plants from maize anther culture. The most common chromosome doubling method applies colchicine to the plants. However, the conventional treatment has several disadvantages. In this experiment, anther cultures of one maize F1 hybrid were treated with 0.02% and 0.03% colchicine for three days at the beginning of microspore induction. Colchicine added to the induction medium had no negative effect on the androgenic response of the genotype examined. The spontaneous doubling rate of the hybrid was very low, underlying the need for an efficient genome doubling system. Significantly higher fertility was observed in plants originating from the colchicine-supplemented induction medium. In conclusion, the results obtained clearly demonstrate that the application of colchicine prior to the first microspore mitosis was superior to the conventionally used chromosome doubling techniques.

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