The regeneration capacity of microspore-derived structures, with various morphological characteristics produced in anther cultures of maize (Zea mays L.) were studied in order to identify the morphotype resulting in the highest yield of spontaneous doubled haploid regenerants. Parallel to the morphological studies the ploidy level of microspore-derived structures and regenerants was analysed by flow cytometry. Neither the growth conditions of the anther donor plants nor the media used in the experiment had any effect on the frequency distribution of different morphotypes. The highest number of spontaneous doubled haploid plants was regenerated from white compact structures 2-3 mm in size, derived from the anthers of phytotron-grown donor plants.
- Microspore-derived structures
- Morphological characteristics
- Regeneration efficiency
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science