Regeneration associated growth factor receptor and epithelial marker expression in lymphoid aggregates of ulcerative colitis

F. Sípos, G. Müzes, Gábor Valcz, O. Galamb, Kinga Tóth, Katalin Leiszter, T. Krenács, Z. Tulassay, B. Molnár

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective. Mesenchymal-epithelial transition may have crucial role in mucosal regeneration, hence we assayed epithelial growth factor receptor (EGFR), insulin-like growth factor receptor-1 (IGF1R), hepatocyte-derived growth factor receptor (HGFR), CDX2 and cytokeratin (CK) expression in lymphoid aggregates (LA) of ulcerative colitis (UC). Material and methods. Tissue microarrays (TMAs) made of biopsy samples from 20 mildly, 20 moderately and 20 severely active UC, 12 non-specific colitis (NSC) and 20 healthy colon were prepared, and immunolabelled with anti-EGFR, -IGF1R, -HGFR, -CDX2, -CK antibodies. After virtual microscopic evaluation, one-way ANOVA and correlation analysis were performed. For validation, TaqMan real-time RT-PCR was performed by using RNA from laser microdissected LA from 10 healthy colon and 10 endoscopically active UC biopsies. Results. The number of LA was in tight positive correlation with the severity of inflammation (r0.9). The number of EGFR/HGFR positive subepithelial cells was found to be significantly elevated in severe (21.6±2.1%/21.3±1.9%), moderate (14.3±1.7%/14.6±1. 6%) and mild (7.2±1.6%/7.4±1.3%) inflammation compared to healthy colon mucosa (2.6±1.4%/2.4±1.03%) (p

Original languageEnglish
Pages (from-to)440-448
Number of pages9
JournalScandinavian Journal of Gastroenterology
Volume45
Issue number4
DOIs
Publication statusPublished - Apr 14 2010

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Proto-Oncogene Proteins c-met
Growth Factor Receptors
Ulcerative Colitis
Regeneration
Colon
Keratins
Inflammation
Somatomedin Receptors
Biopsy
Epithelial-Mesenchymal Transition
Colitis
Real-Time Polymerase Chain Reaction
Analysis of Variance
Mucous Membrane
Lasers
RNA
Antibodies

Keywords

  • CDX2
  • Cytokeratin
  • Growth factor receptor
  • Lymphoid aggregate
  • Mesenchymal-to-epithelial transition
  • Mucosal regeneration
  • Ulcerative colitis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

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title = "Regeneration associated growth factor receptor and epithelial marker expression in lymphoid aggregates of ulcerative colitis",
abstract = "Objective. Mesenchymal-epithelial transition may have crucial role in mucosal regeneration, hence we assayed epithelial growth factor receptor (EGFR), insulin-like growth factor receptor-1 (IGF1R), hepatocyte-derived growth factor receptor (HGFR), CDX2 and cytokeratin (CK) expression in lymphoid aggregates (LA) of ulcerative colitis (UC). Material and methods. Tissue microarrays (TMAs) made of biopsy samples from 20 mildly, 20 moderately and 20 severely active UC, 12 non-specific colitis (NSC) and 20 healthy colon were prepared, and immunolabelled with anti-EGFR, -IGF1R, -HGFR, -CDX2, -CK antibodies. After virtual microscopic evaluation, one-way ANOVA and correlation analysis were performed. For validation, TaqMan real-time RT-PCR was performed by using RNA from laser microdissected LA from 10 healthy colon and 10 endoscopically active UC biopsies. Results. The number of LA was in tight positive correlation with the severity of inflammation (r0.9). The number of EGFR/HGFR positive subepithelial cells was found to be significantly elevated in severe (21.6±2.1{\%}/21.3±1.9{\%}), moderate (14.3±1.7{\%}/14.6±1. 6{\%}) and mild (7.2±1.6{\%}/7.4±1.3{\%}) inflammation compared to healthy colon mucosa (2.6±1.4{\%}/2.4±1.03{\%}) (p",
keywords = "CDX2, Cytokeratin, Growth factor receptor, Lymphoid aggregate, Mesenchymal-to-epithelial transition, Mucosal regeneration, Ulcerative colitis",
author = "F. S{\'i}pos and G. M{\"u}zes and G{\'a}bor Valcz and O. Galamb and Kinga T{\'o}th and Katalin Leiszter and T. Kren{\'a}cs and Z. Tulassay and B. Moln{\'a}r",
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T1 - Regeneration associated growth factor receptor and epithelial marker expression in lymphoid aggregates of ulcerative colitis

AU - Sípos, F.

AU - Müzes, G.

AU - Valcz, Gábor

AU - Galamb, O.

AU - Tóth, Kinga

AU - Leiszter, Katalin

AU - Krenács, T.

AU - Tulassay, Z.

AU - Molnár, B.

PY - 2010/4/14

Y1 - 2010/4/14

N2 - Objective. Mesenchymal-epithelial transition may have crucial role in mucosal regeneration, hence we assayed epithelial growth factor receptor (EGFR), insulin-like growth factor receptor-1 (IGF1R), hepatocyte-derived growth factor receptor (HGFR), CDX2 and cytokeratin (CK) expression in lymphoid aggregates (LA) of ulcerative colitis (UC). Material and methods. Tissue microarrays (TMAs) made of biopsy samples from 20 mildly, 20 moderately and 20 severely active UC, 12 non-specific colitis (NSC) and 20 healthy colon were prepared, and immunolabelled with anti-EGFR, -IGF1R, -HGFR, -CDX2, -CK antibodies. After virtual microscopic evaluation, one-way ANOVA and correlation analysis were performed. For validation, TaqMan real-time RT-PCR was performed by using RNA from laser microdissected LA from 10 healthy colon and 10 endoscopically active UC biopsies. Results. The number of LA was in tight positive correlation with the severity of inflammation (r0.9). The number of EGFR/HGFR positive subepithelial cells was found to be significantly elevated in severe (21.6±2.1%/21.3±1.9%), moderate (14.3±1.7%/14.6±1. 6%) and mild (7.2±1.6%/7.4±1.3%) inflammation compared to healthy colon mucosa (2.6±1.4%/2.4±1.03%) (p

AB - Objective. Mesenchymal-epithelial transition may have crucial role in mucosal regeneration, hence we assayed epithelial growth factor receptor (EGFR), insulin-like growth factor receptor-1 (IGF1R), hepatocyte-derived growth factor receptor (HGFR), CDX2 and cytokeratin (CK) expression in lymphoid aggregates (LA) of ulcerative colitis (UC). Material and methods. Tissue microarrays (TMAs) made of biopsy samples from 20 mildly, 20 moderately and 20 severely active UC, 12 non-specific colitis (NSC) and 20 healthy colon were prepared, and immunolabelled with anti-EGFR, -IGF1R, -HGFR, -CDX2, -CK antibodies. After virtual microscopic evaluation, one-way ANOVA and correlation analysis were performed. For validation, TaqMan real-time RT-PCR was performed by using RNA from laser microdissected LA from 10 healthy colon and 10 endoscopically active UC biopsies. Results. The number of LA was in tight positive correlation with the severity of inflammation (r0.9). The number of EGFR/HGFR positive subepithelial cells was found to be significantly elevated in severe (21.6±2.1%/21.3±1.9%), moderate (14.3±1.7%/14.6±1. 6%) and mild (7.2±1.6%/7.4±1.3%) inflammation compared to healthy colon mucosa (2.6±1.4%/2.4±1.03%) (p

KW - CDX2

KW - Cytokeratin

KW - Growth factor receptor

KW - Lymphoid aggregate

KW - Mesenchymal-to-epithelial transition

KW - Mucosal regeneration

KW - Ulcerative colitis

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