Refractive index of dark-adapted bacteriorhodopsin and tris(hydroxymethyl) aminomethane buffer between 390 and 880 nm

Zsuzsanna Heiner, Károly Osvay

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The refractivity of wild-type bacteriorhodopsin (bRWT) suspended in tris(hydroxymethyl)aminomethane (TRIS) buffer has been measured in the spectral range of 390-840nm by the method of angle of minimal deviation with the use of a hollow glass prism. The refractive indices of pure bRWT as well as of TRIS buffer have been determined from the concentration dependent refraction values. Sellmeier-type dispersion equations have been fitted for both the TRIS buffer and pure bRWT.

Original languageEnglish
Pages (from-to)4610-4615
Number of pages6
JournalApplied Optics
Volume48
Issue number23
DOIs
Publication statusPublished - Aug 10 2009

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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