Refractive index of dark-adapted bacteriorhodopsin and tris(hydroxymethyl) aminomethane buffer between 390 and 880 nm

Zsuzsanna Heiner, K. Osvay

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The refractivity of wild-type bacteriorhodopsin (bRWT) suspended in tris(hydroxymethyl)aminomethane (TRIS) buffer has been measured in the spectral range of 390-840nm by the method of angle of minimal deviation with the use of a hollow glass prism. The refractive indices of pure bRWT as well as of TRIS buffer have been determined from the concentration dependent refraction values. Sellmeier-type dispersion equations have been fitted for both the TRIS buffer and pure bRWT.

Original languageEnglish
Pages (from-to)4610-4615
Number of pages6
JournalApplied Optics
Volume48
Issue number23
DOIs
Publication statusPublished - Aug 10 2009

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Refractive index
buffers
refractivity
Prisms
Refraction
Glass
prisms
refraction
hollow
deviation
glass

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Refractive index of dark-adapted bacteriorhodopsin and tris(hydroxymethyl) aminomethane buffer between 390 and 880 nm. / Heiner, Zsuzsanna; Osvay, K.

In: Applied Optics, Vol. 48, No. 23, 10.08.2009, p. 4610-4615.

Research output: Contribution to journalArticle

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