Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

Pál Vági, Tito Caffi, Kálmán Zoltán Váczy, Márk Z. Németh, Levente Kiss

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.

Original languageEnglish
Pages (from-to)799-802
Number of pages4
JournalEuropean Journal of Plant Pathology
Volume144
Issue number4
DOIs
Publication statusPublished - Apr 1 2016

Keywords

  • Epifluorescence microscopy
  • Ethidium bromide
  • Fluorescein diacetate
  • Grapevine powdery mildew
  • Propidium iodide

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Horticulture

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