Reference materials and representative test materials to develop nanoparticle characterization methods: The NanoChOp project case

Gert Roebben, Vikram Kestens, Z. Varga, Jean Charoud-Got, Yannic Ramaye, Christian Gollwitzer, Dorota Bartczak, Daniel Geißler, James Noble, Stephane Mazoua, Nele Meeus, Philippe Corbisier, Marcell Palmai, J. Mihály, Michael Krumrey, Julie Davies, Ute Resch-Genger, Neelam Kumarswami, Caterina Minelli, Aneta SikoraHeidi Goenaga-Infante

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12 Citations (Scopus)


This paper describes the production and characteristics of the nanoparticle test materials prepared for common use in the collaborative research project NanoChOp (Chemical and optical characterization of nanomaterials in biological systems), in casu suspensions of silica nanoparticles and CdSe/CdS/ZnS quantum dots (QDs). This paper is the first to illustrate how to assess whether nanoparticle test materials meet the requirements of a "reference material" (ISO Guide 30, 2015) or rather those of the recently defined category of "representative test material (RTM)" (ISO/TS 16195, 2013). The NanoChOp test materials were investigated with small-angle X-ray scattering (SAXS), dynamic light scattering (DLS), and centrifugal liquid sedimentation (CLS) to establish whether they complied with the required monomodal particle size distribution. The presence of impurities, aggregates, agglomerates, and viable microorganisms in the suspensions was investigated with DLS, CLS, optical and electron microscopy and via plating on nutrient agar. Suitability of surface functionalization was investigated with attenuated total reflection Fourier transform infrared spectrometry (ATR-FTIR) and via the capacity of the nanoparticles to be fluorescently labeled or to bind antibodies. Between-unit homogeneity and stability were investigated in terms of particle size and zeta potential. This paper shows that only based on the outcome of a detailed characterization process one can raise the status of a test material to RTM or reference material, and how this status depends on its intended use.

Original languageEnglish
Article number56
JournalFrontiers in Chemistry
Issue numberOCT
Publication statusPublished - 2015



  • Analytical quality assurance
  • Materials characterization
  • Metrology
  • Nanoparticle
  • Reference material

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Roebben, G., Kestens, V., Varga, Z., Charoud-Got, J., Ramaye, Y., Gollwitzer, C., Bartczak, D., Geißler, D., Noble, J., Mazoua, S., Meeus, N., Corbisier, P., Palmai, M., Mihály, J., Krumrey, M., Davies, J., Resch-Genger, U., Kumarswami, N., Minelli, C., ... Goenaga-Infante, H. (2015). Reference materials and representative test materials to develop nanoparticle characterization methods: The NanoChOp project case. Frontiers in Chemistry, 3(OCT), [56].