Reduction of apoptosis of in vitro cultured peripheral blood lymphocytes of HIV positive individuals by thymocartin

B. Szende, A. Horvath, K. Nagy, J. Bocsi, I. Kovalszky, E. Barabas

Research output: Contribution to journalArticle

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Abstract

Objective: Apoptotic (natural or programmed) cell death of lymphocytes contributes to an immune decline of HIV-infected persons. Active fragments of thymopoietin may reduce apoptosis and slow down this progress of immune decline. Design: In vitro studies were performed in order to investigate the apoptosis-reducing effect of a new active thymopoietin fragment, Thymocartin (Arg-Lys-Asp-Val). Methods: Separated peripheral blood lymphocytes of 10 asymptomatic HIV-positive and 7 HIV-negative persons were cultured. Unstimulated IL-2-stimulated and IL-2-stimulated plus 0.1 and 0.001 μg/ml Thymocartin-treated cells were investigated for apoptosis by morphological (HE, Apop-Tag), flow cytometric and agarose DNA fragmentation methods. Results: Unstimulated lymphocytes of HIV-positive persons showed higher apoptotic activity than those of HIV-negative persons. IL-2-stimulation increased the apoptotic rate in 7 HIV-positive and 1 HIV-negative individuals. Thymocartin reduced the apoptotic activity in 8 HIV-positive and 1 HIV-negative case. Conclusions: Thymocartin reduces the apoptotic activity in lymphocytes of HIV-positive patients in vitro. This compound may have the same effect in vivo and may prolong the asymptomatic period of HIV-infected patients.

Original languageEnglish
Pages (from-to)456-459
Number of pages4
JournalMedical Science Monitor
Volume3
Issue number4
Publication statusPublished - 1997

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thymocartin
HIV
Lymphocytes
Apoptosis
Thymopoietins
Interleukin-2
HIV-1
In Vitro Techniques
DNA Fragmentation

Keywords

  • Apoptosis
  • HIV-infection
  • Thymocartin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Reduction of apoptosis of in vitro cultured peripheral blood lymphocytes of HIV positive individuals by thymocartin. / Szende, B.; Horvath, A.; Nagy, K.; Bocsi, J.; Kovalszky, I.; Barabas, E.

In: Medical Science Monitor, Vol. 3, No. 4, 1997, p. 456-459.

Research output: Contribution to journalArticle

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T1 - Reduction of apoptosis of in vitro cultured peripheral blood lymphocytes of HIV positive individuals by thymocartin

AU - Szende, B.

AU - Horvath, A.

AU - Nagy, K.

AU - Bocsi, J.

AU - Kovalszky, I.

AU - Barabas, E.

PY - 1997

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N2 - Objective: Apoptotic (natural or programmed) cell death of lymphocytes contributes to an immune decline of HIV-infected persons. Active fragments of thymopoietin may reduce apoptosis and slow down this progress of immune decline. Design: In vitro studies were performed in order to investigate the apoptosis-reducing effect of a new active thymopoietin fragment, Thymocartin (Arg-Lys-Asp-Val). Methods: Separated peripheral blood lymphocytes of 10 asymptomatic HIV-positive and 7 HIV-negative persons were cultured. Unstimulated IL-2-stimulated and IL-2-stimulated plus 0.1 and 0.001 μg/ml Thymocartin-treated cells were investigated for apoptosis by morphological (HE, Apop-Tag), flow cytometric and agarose DNA fragmentation methods. Results: Unstimulated lymphocytes of HIV-positive persons showed higher apoptotic activity than those of HIV-negative persons. IL-2-stimulation increased the apoptotic rate in 7 HIV-positive and 1 HIV-negative individuals. Thymocartin reduced the apoptotic activity in 8 HIV-positive and 1 HIV-negative case. Conclusions: Thymocartin reduces the apoptotic activity in lymphocytes of HIV-positive patients in vitro. This compound may have the same effect in vivo and may prolong the asymptomatic period of HIV-infected patients.

AB - Objective: Apoptotic (natural or programmed) cell death of lymphocytes contributes to an immune decline of HIV-infected persons. Active fragments of thymopoietin may reduce apoptosis and slow down this progress of immune decline. Design: In vitro studies were performed in order to investigate the apoptosis-reducing effect of a new active thymopoietin fragment, Thymocartin (Arg-Lys-Asp-Val). Methods: Separated peripheral blood lymphocytes of 10 asymptomatic HIV-positive and 7 HIV-negative persons were cultured. Unstimulated IL-2-stimulated and IL-2-stimulated plus 0.1 and 0.001 μg/ml Thymocartin-treated cells were investigated for apoptosis by morphological (HE, Apop-Tag), flow cytometric and agarose DNA fragmentation methods. Results: Unstimulated lymphocytes of HIV-positive persons showed higher apoptotic activity than those of HIV-negative persons. IL-2-stimulation increased the apoptotic rate in 7 HIV-positive and 1 HIV-negative individuals. Thymocartin reduced the apoptotic activity in 8 HIV-positive and 1 HIV-negative case. Conclusions: Thymocartin reduces the apoptotic activity in lymphocytes of HIV-positive patients in vitro. This compound may have the same effect in vivo and may prolong the asymptomatic period of HIV-infected patients.

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