Reduction of apoptosis of in vitro cultured lymphocytes of HIV-positive persons by N(G)-hydroxy-methylated-L-arginine and 1'-methyl-ascorbigen

J. Bocsi, K. Nagy, E. Tyihák, L. Trézl, B. Szende

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Some formaldehyde generating chemicals due to reduction of apoptosis in lymphocytes may slow down the progress of immune decline of HIV-infected individuals. N(G)-hydroxy-methylated-L-arginine (MAX) and 1'-methyl-ascorbigen (MeAsc) could enter this way the biochemical pathway of cells and affect the apoptotic process. Separated peripheral blood lymphocytes of five asymptomatic HIV-positive persons were cultured. Unstimulated, IL-2 stimulated and IL-2 stimulated plus 0.1, 1.0, 10.0 μg/ml MAX or MeAsc treated lymphocytes were investigated for apoptosis morphologically (HE) and by flow cytometrical DNA fragmentation method. IL-2 stimulation lowered the apoptotic rate in lymphocytes of HIV-positive persons related to unstimulated ones. MAX and MeAsc reduced the apoptotic activity of stimulated lymphocytes in the least or the middle doses while in the higher dose did not. MAX and MeAsc reduced the apoptotic activity of stimulated lymphocytes originated from HIV-positive patients in vitro. This compounds may have the same effect in vivo and may prolong the symptomless period of HIV-infected patients. The role of methylation and production of formaldehyde in this process is discussed.

Original languageEnglish
Pages (from-to)331-337
Number of pages7
JournalActa Biologica Hungarica
Volume49
Issue number2-4
Publication statusPublished - 1998

Fingerprint

Lymphocytes
apoptosis
human immunodeficiency virus
arginine
Arginine
lymphocytes
HIV
Apoptosis
interleukin-2
Interleukin-2
formaldehyde
Formaldehyde
methylation
Methylation
DNA fragmentation
DNA Fragmentation
dosage
fragmentation
blood
biochemical pathways

Keywords

  • 1'-methyl-ascorbigen
  • Apoptosis
  • Flow cytometry
  • HIV-infection
  • N(G)-hydroxy-methylated-L-arginine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Reduction of apoptosis of in vitro cultured lymphocytes of HIV-positive persons by N(G)-hydroxy-methylated-L-arginine and 1'-methyl-ascorbigen. / Bocsi, J.; Nagy, K.; Tyihák, E.; Trézl, L.; Szende, B.

In: Acta Biologica Hungarica, Vol. 49, No. 2-4, 1998, p. 331-337.

Research output: Contribution to journalArticle

@article{8937efc194ea422593718489b49e1d3e,
title = "Reduction of apoptosis of in vitro cultured lymphocytes of HIV-positive persons by N(G)-hydroxy-methylated-L-arginine and 1'-methyl-ascorbigen",
abstract = "Some formaldehyde generating chemicals due to reduction of apoptosis in lymphocytes may slow down the progress of immune decline of HIV-infected individuals. N(G)-hydroxy-methylated-L-arginine (MAX) and 1'-methyl-ascorbigen (MeAsc) could enter this way the biochemical pathway of cells and affect the apoptotic process. Separated peripheral blood lymphocytes of five asymptomatic HIV-positive persons were cultured. Unstimulated, IL-2 stimulated and IL-2 stimulated plus 0.1, 1.0, 10.0 μg/ml MAX or MeAsc treated lymphocytes were investigated for apoptosis morphologically (HE) and by flow cytometrical DNA fragmentation method. IL-2 stimulation lowered the apoptotic rate in lymphocytes of HIV-positive persons related to unstimulated ones. MAX and MeAsc reduced the apoptotic activity of stimulated lymphocytes in the least or the middle doses while in the higher dose did not. MAX and MeAsc reduced the apoptotic activity of stimulated lymphocytes originated from HIV-positive patients in vitro. This compounds may have the same effect in vivo and may prolong the symptomless period of HIV-infected patients. The role of methylation and production of formaldehyde in this process is discussed.",
keywords = "1'-methyl-ascorbigen, Apoptosis, Flow cytometry, HIV-infection, N(G)-hydroxy-methylated-L-arginine",
author = "J. Bocsi and K. Nagy and E. Tyih{\'a}k and L. Tr{\'e}zl and B. Szende",
year = "1998",
language = "English",
volume = "49",
pages = "331--337",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "2-4",

}

TY - JOUR

T1 - Reduction of apoptosis of in vitro cultured lymphocytes of HIV-positive persons by N(G)-hydroxy-methylated-L-arginine and 1'-methyl-ascorbigen

AU - Bocsi, J.

AU - Nagy, K.

AU - Tyihák, E.

AU - Trézl, L.

AU - Szende, B.

PY - 1998

Y1 - 1998

N2 - Some formaldehyde generating chemicals due to reduction of apoptosis in lymphocytes may slow down the progress of immune decline of HIV-infected individuals. N(G)-hydroxy-methylated-L-arginine (MAX) and 1'-methyl-ascorbigen (MeAsc) could enter this way the biochemical pathway of cells and affect the apoptotic process. Separated peripheral blood lymphocytes of five asymptomatic HIV-positive persons were cultured. Unstimulated, IL-2 stimulated and IL-2 stimulated plus 0.1, 1.0, 10.0 μg/ml MAX or MeAsc treated lymphocytes were investigated for apoptosis morphologically (HE) and by flow cytometrical DNA fragmentation method. IL-2 stimulation lowered the apoptotic rate in lymphocytes of HIV-positive persons related to unstimulated ones. MAX and MeAsc reduced the apoptotic activity of stimulated lymphocytes in the least or the middle doses while in the higher dose did not. MAX and MeAsc reduced the apoptotic activity of stimulated lymphocytes originated from HIV-positive patients in vitro. This compounds may have the same effect in vivo and may prolong the symptomless period of HIV-infected patients. The role of methylation and production of formaldehyde in this process is discussed.

AB - Some formaldehyde generating chemicals due to reduction of apoptosis in lymphocytes may slow down the progress of immune decline of HIV-infected individuals. N(G)-hydroxy-methylated-L-arginine (MAX) and 1'-methyl-ascorbigen (MeAsc) could enter this way the biochemical pathway of cells and affect the apoptotic process. Separated peripheral blood lymphocytes of five asymptomatic HIV-positive persons were cultured. Unstimulated, IL-2 stimulated and IL-2 stimulated plus 0.1, 1.0, 10.0 μg/ml MAX or MeAsc treated lymphocytes were investigated for apoptosis morphologically (HE) and by flow cytometrical DNA fragmentation method. IL-2 stimulation lowered the apoptotic rate in lymphocytes of HIV-positive persons related to unstimulated ones. MAX and MeAsc reduced the apoptotic activity of stimulated lymphocytes in the least or the middle doses while in the higher dose did not. MAX and MeAsc reduced the apoptotic activity of stimulated lymphocytes originated from HIV-positive patients in vitro. This compounds may have the same effect in vivo and may prolong the symptomless period of HIV-infected patients. The role of methylation and production of formaldehyde in this process is discussed.

KW - 1'-methyl-ascorbigen

KW - Apoptosis

KW - Flow cytometry

KW - HIV-infection

KW - N(G)-hydroxy-methylated-L-arginine

UR - http://www.scopus.com/inward/record.url?scp=0032324175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032324175&partnerID=8YFLogxK

M3 - Article

C2 - 10526977

AN - SCOPUS:0032324175

VL - 49

SP - 331

EP - 337

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - 2-4

ER -