Reduction of aflatoxin B1 and Ochratoxin A in cocoa beans infected with Aspergillus via Ergosterol Value

S. O. Aroyeun, G. O. Adegoke, J. Varga, J. Téren

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Aflatoxigenic and ochratoxigenic isolates were used to contaminate cocoa beans in this study and Ergosteol Values (EV) were assessed by means of visual inspection, Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) and Gas Chromatography Mass Spectrometer (GC MS), respectively. Different concentrations of Aframomum danielli essential oils at 500, 1,000, 1,500, and 2,000 ppm were used to treat the infected samples. It was found that aflatoxin B1 (afBSUB align=right1) and Ochratoxin A (OTA) correlated with EV and all correlated with cocoa bean infection and positively r = 0.80 and 0.79. Toxic afBSUB align=right1 in the Aspergillus flavus contaminated cocoa beans reduced from 1.4 ng g10SUP align=right-1/SUP at 1,000 ppm essential oil and reached 0.08 ng g10SUP align=right-1/SUP at 2,000 ppm. The afB1 reduction efficiency with A. danielli was found to be 94.3% while ergosterol reduced by 78.5%. The OTA and the EV of other cocoa bean sample varied according to the contaminating species and its OTA production capacity. The active components of A. danielli such as the mono-terpenes, alkaloids and phenolic acids in the essential oils might have been responsible for the reduction in afBSUB align=right1, OTA and EV as evidenced in this study.

Original languageEnglish
Pages (from-to)75-89
Number of pages15
JournalWorld Review of Science, Technology and Sustainable Development
Volume6
Issue number1
DOIs
Publication statusPublished - 2009

Fingerprint

Cocoa
Ergosterol
Aflatoxin B1
Aspergillus
Volatile Oils
Poisons
Terpenes
High performance liquid chromatography
Mass spectrometers
Alkaloids
Gas chromatography
Inspection
ochratoxin A

Keywords

  • afB
  • Aflatoxin B
  • Aspergillus
  • Cocoa beans
  • Ergosterol
  • Ochratoxin A
  • OTA

ASJC Scopus subject areas

  • General

Cite this

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title = "Reduction of aflatoxin B1 and Ochratoxin A in cocoa beans infected with Aspergillus via Ergosterol Value",
abstract = "Aflatoxigenic and ochratoxigenic isolates were used to contaminate cocoa beans in this study and Ergosteol Values (EV) were assessed by means of visual inspection, Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) and Gas Chromatography Mass Spectrometer (GC MS), respectively. Different concentrations of Aframomum danielli essential oils at 500, 1,000, 1,500, and 2,000 ppm were used to treat the infected samples. It was found that aflatoxin B1 (afBSUB align=right1) and Ochratoxin A (OTA) correlated with EV and all correlated with cocoa bean infection and positively r = 0.80 and 0.79. Toxic afBSUB align=right1 in the Aspergillus flavus contaminated cocoa beans reduced from 1.4 ng g10SUP align=right-1/SUP at 1,000 ppm essential oil and reached 0.08 ng g10SUP align=right-1/SUP at 2,000 ppm. The afB1 reduction efficiency with A. danielli was found to be 94.3{\%} while ergosterol reduced by 78.5{\%}. The OTA and the EV of other cocoa bean sample varied according to the contaminating species and its OTA production capacity. The active components of A. danielli such as the mono-terpenes, alkaloids and phenolic acids in the essential oils might have been responsible for the reduction in afBSUB align=right1, OTA and EV as evidenced in this study.",
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author = "Aroyeun, {S. O.} and Adegoke, {G. O.} and J. Varga and J. T{\'e}ren",
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T1 - Reduction of aflatoxin B1 and Ochratoxin A in cocoa beans infected with Aspergillus via Ergosterol Value

AU - Aroyeun, S. O.

AU - Adegoke, G. O.

AU - Varga, J.

AU - Téren, J.

PY - 2009

Y1 - 2009

N2 - Aflatoxigenic and ochratoxigenic isolates were used to contaminate cocoa beans in this study and Ergosteol Values (EV) were assessed by means of visual inspection, Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) and Gas Chromatography Mass Spectrometer (GC MS), respectively. Different concentrations of Aframomum danielli essential oils at 500, 1,000, 1,500, and 2,000 ppm were used to treat the infected samples. It was found that aflatoxin B1 (afBSUB align=right1) and Ochratoxin A (OTA) correlated with EV and all correlated with cocoa bean infection and positively r = 0.80 and 0.79. Toxic afBSUB align=right1 in the Aspergillus flavus contaminated cocoa beans reduced from 1.4 ng g10SUP align=right-1/SUP at 1,000 ppm essential oil and reached 0.08 ng g10SUP align=right-1/SUP at 2,000 ppm. The afB1 reduction efficiency with A. danielli was found to be 94.3% while ergosterol reduced by 78.5%. The OTA and the EV of other cocoa bean sample varied according to the contaminating species and its OTA production capacity. The active components of A. danielli such as the mono-terpenes, alkaloids and phenolic acids in the essential oils might have been responsible for the reduction in afBSUB align=right1, OTA and EV as evidenced in this study.

AB - Aflatoxigenic and ochratoxigenic isolates were used to contaminate cocoa beans in this study and Ergosteol Values (EV) were assessed by means of visual inspection, Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) and Gas Chromatography Mass Spectrometer (GC MS), respectively. Different concentrations of Aframomum danielli essential oils at 500, 1,000, 1,500, and 2,000 ppm were used to treat the infected samples. It was found that aflatoxin B1 (afBSUB align=right1) and Ochratoxin A (OTA) correlated with EV and all correlated with cocoa bean infection and positively r = 0.80 and 0.79. Toxic afBSUB align=right1 in the Aspergillus flavus contaminated cocoa beans reduced from 1.4 ng g10SUP align=right-1/SUP at 1,000 ppm essential oil and reached 0.08 ng g10SUP align=right-1/SUP at 2,000 ppm. The afB1 reduction efficiency with A. danielli was found to be 94.3% while ergosterol reduced by 78.5%. The OTA and the EV of other cocoa bean sample varied according to the contaminating species and its OTA production capacity. The active components of A. danielli such as the mono-terpenes, alkaloids and phenolic acids in the essential oils might have been responsible for the reduction in afBSUB align=right1, OTA and EV as evidenced in this study.

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