Abstract
Abiotic stresses induce oxidative stress, which modifies the level of several metabolites including amino acids. The redox control of free amino acid profile was monitored in wild-type and ascorbate or glutathione deficient mutant Arabidopsis thaliana plants before and after hydroponic treatment with various redox agents. Both mutations and treatments modified the size and redox state of the ascorbate (AsA) and/or glutathione (GSH) pools. The total free amino acid content was increased by AsA, GSH and H2O2 in all three genotypes and a very large (threefold) increase was observed in the GSH-deficient pad2-1 mutant after GSH treatment compared with the untreated wild-type plants. Addition of GSH reduced the ratio of amino acids belonging to the glutamate family on a large scale and increased the relative amount of non-proteinogenic amino acids. The latter change was because of the large increase in the content of alpha-aminoadipate, an inhibitor of glutamatic acid (Glu) transport. Most of the treatments increased the proline (Pro) content, which effect was due to the activation of genes involved in Pro synthesis. Although all studied redox compounds influenced the amount of free amino acids and a mostly positive, very close (r > 0.9) correlation exists between these parameters, a special regulatory role of GSH could be presumed due to its more powerful effect. This may originate from the thiol/disulphide conversion or (de)glutathionylation of enzymes participating in the amino acid metabolism.
| Original language | English |
|---|---|
| Pages (from-to) | 264-276 |
| Number of pages | 13 |
| Journal | Physiologia Plantarum |
| Volume | 159 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 1 2017 |
Fingerprint
ASJC Scopus subject areas
- Physiology
- Genetics
- Plant Science
- Cell Biology
Cite this
Redox regulation of free amino acid levels in Arabidopsis thaliana. / Gulyás, Zsolt; Simon-Sarkadi, L.; Badics, Eszter; Novák, Aliz; Mednyánszky, Zsuzsanna; Szalai, G.; Galiba, G.; Kocsy, G.
In: Physiologia Plantarum, Vol. 159, No. 3, 01.03.2017, p. 264-276.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Redox regulation of free amino acid levels in Arabidopsis thaliana
AU - Gulyás, Zsolt
AU - Simon-Sarkadi, L.
AU - Badics, Eszter
AU - Novák, Aliz
AU - Mednyánszky, Zsuzsanna
AU - Szalai, G.
AU - Galiba, G.
AU - Kocsy, G.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Abiotic stresses induce oxidative stress, which modifies the level of several metabolites including amino acids. The redox control of free amino acid profile was monitored in wild-type and ascorbate or glutathione deficient mutant Arabidopsis thaliana plants before and after hydroponic treatment with various redox agents. Both mutations and treatments modified the size and redox state of the ascorbate (AsA) and/or glutathione (GSH) pools. The total free amino acid content was increased by AsA, GSH and H2O2 in all three genotypes and a very large (threefold) increase was observed in the GSH-deficient pad2-1 mutant after GSH treatment compared with the untreated wild-type plants. Addition of GSH reduced the ratio of amino acids belonging to the glutamate family on a large scale and increased the relative amount of non-proteinogenic amino acids. The latter change was because of the large increase in the content of alpha-aminoadipate, an inhibitor of glutamatic acid (Glu) transport. Most of the treatments increased the proline (Pro) content, which effect was due to the activation of genes involved in Pro synthesis. Although all studied redox compounds influenced the amount of free amino acids and a mostly positive, very close (r > 0.9) correlation exists between these parameters, a special regulatory role of GSH could be presumed due to its more powerful effect. This may originate from the thiol/disulphide conversion or (de)glutathionylation of enzymes participating in the amino acid metabolism.
AB - Abiotic stresses induce oxidative stress, which modifies the level of several metabolites including amino acids. The redox control of free amino acid profile was monitored in wild-type and ascorbate or glutathione deficient mutant Arabidopsis thaliana plants before and after hydroponic treatment with various redox agents. Both mutations and treatments modified the size and redox state of the ascorbate (AsA) and/or glutathione (GSH) pools. The total free amino acid content was increased by AsA, GSH and H2O2 in all three genotypes and a very large (threefold) increase was observed in the GSH-deficient pad2-1 mutant after GSH treatment compared with the untreated wild-type plants. Addition of GSH reduced the ratio of amino acids belonging to the glutamate family on a large scale and increased the relative amount of non-proteinogenic amino acids. The latter change was because of the large increase in the content of alpha-aminoadipate, an inhibitor of glutamatic acid (Glu) transport. Most of the treatments increased the proline (Pro) content, which effect was due to the activation of genes involved in Pro synthesis. Although all studied redox compounds influenced the amount of free amino acids and a mostly positive, very close (r > 0.9) correlation exists between these parameters, a special regulatory role of GSH could be presumed due to its more powerful effect. This may originate from the thiol/disulphide conversion or (de)glutathionylation of enzymes participating in the amino acid metabolism.
UR - http://www.scopus.com/inward/record.url?scp=84990059605&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84990059605&partnerID=8YFLogxK
U2 - 10.1111/ppl.12510
DO - 10.1111/ppl.12510
M3 - Article
C2 - 27605256
AN - SCOPUS:84990059605
VL - 159
SP - 264
EP - 276
JO - Physiologia Plantarum
JF - Physiologia Plantarum
SN - 0031-9317
IS - 3
ER -