Redox regulation of free amino acid levels in Arabidopsis thaliana

Zsolt Gulyás, L. Simon-Sarkadi, Eszter Badics, Aliz Novák, Zsuzsanna Mednyánszky, G. Szalai, G. Galiba, G. Kocsy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Abiotic stresses induce oxidative stress, which modifies the level of several metabolites including amino acids. The redox control of free amino acid profile was monitored in wild-type and ascorbate or glutathione deficient mutant Arabidopsis thaliana plants before and after hydroponic treatment with various redox agents. Both mutations and treatments modified the size and redox state of the ascorbate (AsA) and/or glutathione (GSH) pools. The total free amino acid content was increased by AsA, GSH and H2O2 in all three genotypes and a very large (threefold) increase was observed in the GSH-deficient pad2-1 mutant after GSH treatment compared with the untreated wild-type plants. Addition of GSH reduced the ratio of amino acids belonging to the glutamate family on a large scale and increased the relative amount of non-proteinogenic amino acids. The latter change was because of the large increase in the content of alpha-aminoadipate, an inhibitor of glutamatic acid (Glu) transport. Most of the treatments increased the proline (Pro) content, which effect was due to the activation of genes involved in Pro synthesis. Although all studied redox compounds influenced the amount of free amino acids and a mostly positive, very close (r > 0.9) correlation exists between these parameters, a special regulatory role of GSH could be presumed due to its more powerful effect. This may originate from the thiol/disulphide conversion or (de)glutathionylation of enzymes participating in the amino acid metabolism.

Original languageEnglish
Pages (from-to)264-276
Number of pages13
JournalPhysiologia Plantarum
Volume159
Issue number3
DOIs
Publication statusPublished - Mar 1 2017

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Arabidopsis
free amino acids
Oxidation-Reduction
Arabidopsis thaliana
Amino Acids
amino acids
proline
glutathione
mutants
gene activation
amino acid metabolism
thiols
sulfides
hydroponics
glutamates
abiotic stress
Proline
oxidative stress
Glutathione
metabolites

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Redox regulation of free amino acid levels in Arabidopsis thaliana. / Gulyás, Zsolt; Simon-Sarkadi, L.; Badics, Eszter; Novák, Aliz; Mednyánszky, Zsuzsanna; Szalai, G.; Galiba, G.; Kocsy, G.

In: Physiologia Plantarum, Vol. 159, No. 3, 01.03.2017, p. 264-276.

Research output: Contribution to journalArticle

Gulyás, Zsolt ; Simon-Sarkadi, L. ; Badics, Eszter ; Novák, Aliz ; Mednyánszky, Zsuzsanna ; Szalai, G. ; Galiba, G. ; Kocsy, G. / Redox regulation of free amino acid levels in Arabidopsis thaliana. In: Physiologia Plantarum. 2017 ; Vol. 159, No. 3. pp. 264-276.
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