Recycling of bowel content: The importance of the right timing

Istvan Pataki, Judit Szabo, Petra Varga, Andrea Berkes, Andrea Nagy, Fiona Murphy, Antonino Morabito, George Rakoczy, Tamas Cserni

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Introduction: Extracorporeal stool transport (recycling of chyme discharged from the proximal stoma end to the distal end of a high jejunostomy or ileostomy) is thought to be beneficial in preventing malabsoprtion, sodium loss, cholestasis and atrophy of the distal intestine until restoration of the intestinal continuity becomes possible. However little is known about its adverse effects. Our aim was to investigate the microbiological safety of recycling. Material and Method: Native samples were taken from the proximal stoma in 5 premature neonates who underwent an ileostomy or a jejunostomy due to necrotising enterocolitis, for qualitative culture. The first sample was drawn immediately after the change of the stoma bag, further samples were sent from the stoma bag at 30, 60, 90, 120, 150, and 180 min later. The samples were inoculated by calibrated (10 μl) loops onto blood agar (5% sheep blood), eosin-methylene blue agar and anaerobic blood agar, respectively (Oxoid). The aerobic plates were incubated for 18-20 h at 5% CO2, whereas the anaerobic plates were incubated for 24-48 h in an anaerobic chamber (Concept 400). The bacterial strains were identified to species level by specific biochemical reactions, RapID-ANA II system (Oxoid) and ID32E, Rapid ID 32 Strep ATB automatic system cards (bioMérieux). Results: The number of colony forming unit (CFU) of Gram-negative bacteria (mainly E. coli) exponentially increased after 30 min and reached 105/ml after 120 min. Gram-positive strains (primarily E. faecalis) were detected after 60 min and CFU increased to 105/ml after 120 min. The number of anaerobic (principally Bacteroides fragilis) CFU started to increase after 120 min. In two cases coagulase negative Staphylococcus strains were isolated the earliest in the chyme. The average of total CFU approached 105/ml after 90 min and exceeded 105/ml after 120 min. Conclusion: The chyme in the stoma bag is colonized by commensal facultative pathogenic enteral/colonic as well as skin flora species after 120 min. Recycling of stoma bag content may be dangerous after 90 min.

Original languageEnglish
Pages (from-to)579-584
Number of pages6
JournalJournal of Pediatric Surgery
Volume48
Issue number3
DOIs
Publication statusPublished - Mar 1 2013

Keywords

  • Extracorporeal stool transport
  • Necrotising enterocolitis
  • Recycling
  • Short bowel syndrome

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

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  • Cite this

    Pataki, I., Szabo, J., Varga, P., Berkes, A., Nagy, A., Murphy, F., Morabito, A., Rakoczy, G., & Cserni, T. (2013). Recycling of bowel content: The importance of the right timing. Journal of Pediatric Surgery, 48(3), 579-584. https://doi.org/10.1016/j.jpedsurg.2012.07.064