Recruiting new neurons from the subventricular zone to the rat postnatal cortex: An organotypic slice culture model

A. G. Dayer, B. Jenny, G. Potter, M. O. Sauvain, G. Szabó, L. Vutskits, E. Gascon, J. Kiss

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The neurogenic subventricular zone (SVZ) of the lateral ventricle is a potential source for neuronal replacement in the postnatal or adult neocortex after injury. Here we present a novel model system to directly explore the cellular mechanisms of this process. In order to visualize directed migration from the SVZ towards the cortex, we transplanted green fluorescent protein-labeled progenitor/stem cells into the SVZ of newborn rats. At 2 days after transplantation, we generated organotypic slice cultures and applied fluorescent time-lapse imaging to explore directly the migration and integration of donor cells into the host tissue for up to 2 weeks. Our studies revealed that subventricular grafts provide a significant number of immature neurons to neocortical regions. In the cortex, immature neurons first migrate radially towards the pial surface and then differentiate into GABAergic interneurons. We conclude that our model system presents a novel and effective experimental paradigm to evaluate the recruitment of SVZ-derived neurons into the postnatal cortex, a phenomenon that may represent a potential route for cortical repair.

Original languageEnglish
Pages (from-to)1051-1060
Number of pages10
JournalEuropean Journal of Neuroscience
Volume27
Issue number5
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Lateral Ventricles
Neurons
Stem Cells
Time-Lapse Imaging
Neocortex
Interneurons
Green Fluorescent Proteins
Transplantation
Transplants
Wounds and Injuries

Keywords

  • Brain repair
  • Cell migration
  • Neural progenitors
  • Organotypic
  • Transplantation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Recruiting new neurons from the subventricular zone to the rat postnatal cortex : An organotypic slice culture model. / Dayer, A. G.; Jenny, B.; Potter, G.; Sauvain, M. O.; Szabó, G.; Vutskits, L.; Gascon, E.; Kiss, J.

In: European Journal of Neuroscience, Vol. 27, No. 5, 03.2008, p. 1051-1060.

Research output: Contribution to journalArticle

Dayer, A. G. ; Jenny, B. ; Potter, G. ; Sauvain, M. O. ; Szabó, G. ; Vutskits, L. ; Gascon, E. ; Kiss, J. / Recruiting new neurons from the subventricular zone to the rat postnatal cortex : An organotypic slice culture model. In: European Journal of Neuroscience. 2008 ; Vol. 27, No. 5. pp. 1051-1060.
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