Recovery of protein and chlorophyll from alfalfa by simultaneous lactic acid fermentation and enzyme hydrolysis (ENLAC)

Z. G. Weinberg, G. Szakács, J. C. Linden, R. P. Tengerdy

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Simultaneous lactic acid fermentation and enzyme hydrolysis by cell wall degrading enzymes (ENLAC for short) was tested for improving the recovery of cell content, such as protein and chlorophyll, from alfalfa. Alfalfa was ensiled with the addition of 1.0% (wet weight) of a variety of commercial enzymes or enzyme cocktails. The best result was achieved by the addition of a 1:1 mixture of Novo Viscozyme (containing mainly hemicellulases, cellulases, and pectinases) and Novo Celluclast 1.5 L or Genencore Cellulase 150 L (containing T. reesei cellulases). ENLAC improved the recovery of protein by 160%, chlorophyll by 240%. The same enzyme treatment in a 24-h reaction without ensiling resulted in only a 14% increase in protein recovery. ENLAC provided optimal acidic conditions for enzyme action, and due to the preservation of the plant material during ensiling, it made possible the efficient use of a low concentration of enzymes during a longer reaction time, compared to conventional 24-h enzyme treatments.

Original languageEnglish
Pages (from-to)921-925
Number of pages5
JournalEnzyme and Microbial Technology
Volume12
Issue number12
DOIs
Publication statusPublished - 1990

Fingerprint

Medicago sativa
Chlorophyll
Lactic acid
Fermentation
Hydrolysis
Lactic Acid
Enzymes
Proteins
Recovery
Cellulases
Cellulase
Cell Wall
Cells
Weights and Measures

Keywords

  • alfalfa silage
  • ENLAC
  • plant cell wall degrading enzymes
  • protein and chlorophyll recovery

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Recovery of protein and chlorophyll from alfalfa by simultaneous lactic acid fermentation and enzyme hydrolysis (ENLAC). / Weinberg, Z. G.; Szakács, G.; Linden, J. C.; Tengerdy, R. P.

In: Enzyme and Microbial Technology, Vol. 12, No. 12, 1990, p. 921-925.

Research output: Contribution to journalArticle

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