Recovery of Dunaliella salina cells following hydrogenation of lipids in specific membranes by a homogeneous palladium catalyst

L. Vígh, I. Horváth, Guy A. Thompson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Unsaturated fatty acyl chains of Dunaliella salina membrane lipids can be catalytically reduced by the homogeneous hydrogenation catalyst palladium di(sodium alizarine monosulphonate), Pd(QS)2, under conditions permitting full recovery of the cells within 24 h. The hydrogenation is accomplished by incubation of cells with the hydride form of Pd(QS)2 under 1 atmosphere of H2 and for 2 min or less. Following this protocol, hydrogenation reduces only those fatty acids located in the plasma membrane and other membranes located near the cell surface. The limited reactivity in vivo is due to the fact the Pd(QS)2 permeates into the living cells more slowly than it does into liposomes prepared from extracted Dunaliella membrane lipids. While Dunaliella is completely unaffected by exposure to the oxygenated, inactive catalyst, hydrogenated cells cease growth for approximately 12 h, during which time the hydrogenated acyl chains are being enzymatically retroconverted to their original unsaturated form. When the lipid composition approaches its prehydrogenation values, growth resumes, presumably due to the restoration of normal membrane functions. The system shows promise for studying the metabolic regulation of membrane microviscosity.

Original languageEnglish
Pages (from-to)42-50
Number of pages9
JournalBBA - Biomembranes
Volume937
Issue numberC
DOIs
Publication statusPublished - 1988

Fingerprint

Hydrogenation
Palladium
Membranes
Lipids
Recovery
Catalysts
Membrane Lipids
Cells
Cell growth
Cell membranes
Hydrides
Liposomes
Restoration
Growth
Atmosphere
Fatty Acids
Cell Membrane
Salinum
palladium sulfonated alizarine
Chemical analysis

Keywords

  • (D. salina)
  • Fluidity regulation
  • Hydrogenation catalyst
  • Lipid hydrogenation
  • Unsaturated fatty acid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Cite this

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abstract = "Unsaturated fatty acyl chains of Dunaliella salina membrane lipids can be catalytically reduced by the homogeneous hydrogenation catalyst palladium di(sodium alizarine monosulphonate), Pd(QS)2, under conditions permitting full recovery of the cells within 24 h. The hydrogenation is accomplished by incubation of cells with the hydride form of Pd(QS)2 under 1 atmosphere of H2 and for 2 min or less. Following this protocol, hydrogenation reduces only those fatty acids located in the plasma membrane and other membranes located near the cell surface. The limited reactivity in vivo is due to the fact the Pd(QS)2 permeates into the living cells more slowly than it does into liposomes prepared from extracted Dunaliella membrane lipids. While Dunaliella is completely unaffected by exposure to the oxygenated, inactive catalyst, hydrogenated cells cease growth for approximately 12 h, during which time the hydrogenated acyl chains are being enzymatically retroconverted to their original unsaturated form. When the lipid composition approaches its prehydrogenation values, growth resumes, presumably due to the restoration of normal membrane functions. The system shows promise for studying the metabolic regulation of membrane microviscosity.",
keywords = "(D. salina), Fluidity regulation, Hydrogenation catalyst, Lipid hydrogenation, Unsaturated fatty acid",
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T1 - Recovery of Dunaliella salina cells following hydrogenation of lipids in specific membranes by a homogeneous palladium catalyst

AU - Vígh, L.

AU - Horváth, I.

AU - Thompson, Guy A.

PY - 1988

Y1 - 1988

N2 - Unsaturated fatty acyl chains of Dunaliella salina membrane lipids can be catalytically reduced by the homogeneous hydrogenation catalyst palladium di(sodium alizarine monosulphonate), Pd(QS)2, under conditions permitting full recovery of the cells within 24 h. The hydrogenation is accomplished by incubation of cells with the hydride form of Pd(QS)2 under 1 atmosphere of H2 and for 2 min or less. Following this protocol, hydrogenation reduces only those fatty acids located in the plasma membrane and other membranes located near the cell surface. The limited reactivity in vivo is due to the fact the Pd(QS)2 permeates into the living cells more slowly than it does into liposomes prepared from extracted Dunaliella membrane lipids. While Dunaliella is completely unaffected by exposure to the oxygenated, inactive catalyst, hydrogenated cells cease growth for approximately 12 h, during which time the hydrogenated acyl chains are being enzymatically retroconverted to their original unsaturated form. When the lipid composition approaches its prehydrogenation values, growth resumes, presumably due to the restoration of normal membrane functions. The system shows promise for studying the metabolic regulation of membrane microviscosity.

AB - Unsaturated fatty acyl chains of Dunaliella salina membrane lipids can be catalytically reduced by the homogeneous hydrogenation catalyst palladium di(sodium alizarine monosulphonate), Pd(QS)2, under conditions permitting full recovery of the cells within 24 h. The hydrogenation is accomplished by incubation of cells with the hydride form of Pd(QS)2 under 1 atmosphere of H2 and for 2 min or less. Following this protocol, hydrogenation reduces only those fatty acids located in the plasma membrane and other membranes located near the cell surface. The limited reactivity in vivo is due to the fact the Pd(QS)2 permeates into the living cells more slowly than it does into liposomes prepared from extracted Dunaliella membrane lipids. While Dunaliella is completely unaffected by exposure to the oxygenated, inactive catalyst, hydrogenated cells cease growth for approximately 12 h, during which time the hydrogenated acyl chains are being enzymatically retroconverted to their original unsaturated form. When the lipid composition approaches its prehydrogenation values, growth resumes, presumably due to the restoration of normal membrane functions. The system shows promise for studying the metabolic regulation of membrane microviscosity.

KW - (D. salina)

KW - Fluidity regulation

KW - Hydrogenation catalyst

KW - Lipid hydrogenation

KW - Unsaturated fatty acid

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