Recovery of a recombinant thermostable endoglucanase from E. Coli using supercritical carbon dioxide cell disruption

T. Juhász, E. Székely, B. Simándi, Zs. Szengyel, K. Réczey

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

For the production of thermostable endoglucanase from Clostridium thermocellulum the celC gene was cloned into Escherichia coli BL 21 (DE3) host. The recombinant E. coli was grown in shake flask cultures. The intracellular recombinant protein was extracted from the cells after applying supercritical CO2 cell disruption. The supercritical CO2 cell disintegration was optimized and then compared to the traditional ultrasonic cell disruption technique. With the supercritical cell disruption the cellulase recovery was approximately 17% lower than that of obtained with sonication.

Original languageEnglish
Pages (from-to)131-134
Number of pages4
JournalChemical and Biochemical Engineering Quarterly
Volume17
Issue number2
Publication statusPublished - Jun 2003

Fingerprint

Cellulase
Carbon Dioxide
Escherichia coli
Carbon dioxide
Recovery
Recombinant proteins
Clostridium
Sonication
Disintegration
Recombinant Proteins
Genes
Ultrasonics
Batch Cell Culture Techniques

Keywords

  • Cell disintegration
  • Clostridium thermocellum
  • Recombinant E. coli
  • Sonication
  • Supercritical carbon dioxide
  • Thermostable endoglucanase

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Biotechnology

Cite this

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T1 - Recovery of a recombinant thermostable endoglucanase from E. Coli using supercritical carbon dioxide cell disruption

AU - Juhász, T.

AU - Székely, E.

AU - Simándi, B.

AU - Szengyel, Zs.

AU - Réczey, K.

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AB - For the production of thermostable endoglucanase from Clostridium thermocellulum the celC gene was cloned into Escherichia coli BL 21 (DE3) host. The recombinant E. coli was grown in shake flask cultures. The intracellular recombinant protein was extracted from the cells after applying supercritical CO2 cell disruption. The supercritical CO2 cell disintegration was optimized and then compared to the traditional ultrasonic cell disruption technique. With the supercritical cell disruption the cellulase recovery was approximately 17% lower than that of obtained with sonication.

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KW - Recombinant E. coli

KW - Sonication

KW - Supercritical carbon dioxide

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