Recovery from immobilisation: Responses of fast-twitch muscle fibres to spontaneous and intensive exercise in rat calf muscles

M. Venojärvi, M. Kvist, M. Atalay, L. Józsa, H. Kalimo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Four weeks of immobilisation with two types of re-mobilisation programmes (intensive concentric treadmill exercising during 6 days, and free exercising, and immobilisation without any re-mobilisation period were studied to clarify possible exercise-induced calf muscle damage especially in fast-twitch fibres used in running compared to non-immobilised rats housing freely in their cages. As markers of muscle injury, conventional histology, β-glucuronidase (β-GU) activity and fetal myosin heavy chain expression (MHC-d) were assessed on Days 0, 1, 3, 6 and 14 after the cast removal. Only minor focal hypercontraction, ruptures and necrosis of myofibrils, and weak inflammatory cell reactions were found in all samples examined, except in the controls. No MHC-d positive cells were found indicating absence of active regeneration after immobilisation or re-mobilisation. Minor increase in β-GU activity was observed in all three muscles studied, but statistically significant increase was observed only in the samples of the free exercising group on Day 14 after the cast removal. To conclude, intensive concentric treadmill exercise for 6 days did not cause significantly more muscle damage than did free exercising re-mobilisation.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalPathophysiology
Volume11
Issue number1
DOIs
Publication statusPublished - Jul 2004

Fingerprint

Fast-Twitch Muscle Fibers
Immobilization
Muscles
Fetal Movement
Myosin Heavy Chains
Myofibrils
Glucuronidase
Running
Regeneration
Rupture
Histology
Necrosis
Wounds and Injuries

Keywords

  • Acute exercise
  • Exhaustive exercise
  • Mobilisation
  • Muscle damage
  • Skeletal muscle

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Recovery from immobilisation : Responses of fast-twitch muscle fibres to spontaneous and intensive exercise in rat calf muscles. / Venojärvi, M.; Kvist, M.; Atalay, M.; Józsa, L.; Kalimo, H.

In: Pathophysiology, Vol. 11, No. 1, 07.2004, p. 17-22.

Research output: Contribution to journalArticle

@article{3c1d1bd9823c42dfa36b6c0d1e5f134e,
title = "Recovery from immobilisation: Responses of fast-twitch muscle fibres to spontaneous and intensive exercise in rat calf muscles",
abstract = "Four weeks of immobilisation with two types of re-mobilisation programmes (intensive concentric treadmill exercising during 6 days, and free exercising, and immobilisation without any re-mobilisation period were studied to clarify possible exercise-induced calf muscle damage especially in fast-twitch fibres used in running compared to non-immobilised rats housing freely in their cages. As markers of muscle injury, conventional histology, β-glucuronidase (β-GU) activity and fetal myosin heavy chain expression (MHC-d) were assessed on Days 0, 1, 3, 6 and 14 after the cast removal. Only minor focal hypercontraction, ruptures and necrosis of myofibrils, and weak inflammatory cell reactions were found in all samples examined, except in the controls. No MHC-d positive cells were found indicating absence of active regeneration after immobilisation or re-mobilisation. Minor increase in β-GU activity was observed in all three muscles studied, but statistically significant increase was observed only in the samples of the free exercising group on Day 14 after the cast removal. To conclude, intensive concentric treadmill exercise for 6 days did not cause significantly more muscle damage than did free exercising re-mobilisation.",
keywords = "Acute exercise, Exhaustive exercise, Mobilisation, Muscle damage, Skeletal muscle",
author = "M. Venoj{\"a}rvi and M. Kvist and M. Atalay and L. J{\'o}zsa and H. Kalimo",
year = "2004",
month = "7",
doi = "10.1016/j.pathophys.2003.10.002",
language = "English",
volume = "11",
pages = "17--22",
journal = "Pathophysiology",
issn = "0928-4680",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Recovery from immobilisation

T2 - Responses of fast-twitch muscle fibres to spontaneous and intensive exercise in rat calf muscles

AU - Venojärvi, M.

AU - Kvist, M.

AU - Atalay, M.

AU - Józsa, L.

AU - Kalimo, H.

PY - 2004/7

Y1 - 2004/7

N2 - Four weeks of immobilisation with two types of re-mobilisation programmes (intensive concentric treadmill exercising during 6 days, and free exercising, and immobilisation without any re-mobilisation period were studied to clarify possible exercise-induced calf muscle damage especially in fast-twitch fibres used in running compared to non-immobilised rats housing freely in their cages. As markers of muscle injury, conventional histology, β-glucuronidase (β-GU) activity and fetal myosin heavy chain expression (MHC-d) were assessed on Days 0, 1, 3, 6 and 14 after the cast removal. Only minor focal hypercontraction, ruptures and necrosis of myofibrils, and weak inflammatory cell reactions were found in all samples examined, except in the controls. No MHC-d positive cells were found indicating absence of active regeneration after immobilisation or re-mobilisation. Minor increase in β-GU activity was observed in all three muscles studied, but statistically significant increase was observed only in the samples of the free exercising group on Day 14 after the cast removal. To conclude, intensive concentric treadmill exercise for 6 days did not cause significantly more muscle damage than did free exercising re-mobilisation.

AB - Four weeks of immobilisation with two types of re-mobilisation programmes (intensive concentric treadmill exercising during 6 days, and free exercising, and immobilisation without any re-mobilisation period were studied to clarify possible exercise-induced calf muscle damage especially in fast-twitch fibres used in running compared to non-immobilised rats housing freely in their cages. As markers of muscle injury, conventional histology, β-glucuronidase (β-GU) activity and fetal myosin heavy chain expression (MHC-d) were assessed on Days 0, 1, 3, 6 and 14 after the cast removal. Only minor focal hypercontraction, ruptures and necrosis of myofibrils, and weak inflammatory cell reactions were found in all samples examined, except in the controls. No MHC-d positive cells were found indicating absence of active regeneration after immobilisation or re-mobilisation. Minor increase in β-GU activity was observed in all three muscles studied, but statistically significant increase was observed only in the samples of the free exercising group on Day 14 after the cast removal. To conclude, intensive concentric treadmill exercise for 6 days did not cause significantly more muscle damage than did free exercising re-mobilisation.

KW - Acute exercise

KW - Exhaustive exercise

KW - Mobilisation

KW - Muscle damage

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=2642511333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2642511333&partnerID=8YFLogxK

U2 - 10.1016/j.pathophys.2003.10.002

DO - 10.1016/j.pathophys.2003.10.002

M3 - Article

AN - SCOPUS:2642511333

VL - 11

SP - 17

EP - 22

JO - Pathophysiology

JF - Pathophysiology

SN - 0928-4680

IS - 1

ER -