Recombination of baculovirus DNA following lipofection of insect larvae

J. P. Hajós, D. Zuidema, P. Kulcsár, J. G M Heldens, P. Závodszky, J. M. Vlak

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

By injection of a liposomal complex containing baculovirus DNA directly into the hemocoel of insect larvae a polyhedrosis disease was induced. After cotransfection of insect larvae with circular viral DNA and transfer vector DNA recombinant viruses were generated with a frequency of about 2%, similar to what is obtained in vitro using insect cell cultures. Based on these results an alternative strategy for the generation of recombinants can be derived for baculoviruses for which susceptible cell lines are not readily available. This strategy involves the injection of baculovirus DNA into susceptible larvae followed by in vivo cloning.

Original languageEnglish
Pages (from-to)2045-2050
Number of pages6
JournalArchives of Virology
Volume143
Issue number10
DOIs
Publication statusPublished - 1998

Fingerprint

Baculoviridae
Genetic Recombination
Larva
Insects
DNA
Circular DNA
Injections
DNA Viruses
Viral DNA
Organism Cloning
Cell Culture Techniques
Cell Line

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Hajós, J. P., Zuidema, D., Kulcsár, P., Heldens, J. G. M., Závodszky, P., & Vlak, J. M. (1998). Recombination of baculovirus DNA following lipofection of insect larvae. Archives of Virology, 143(10), 2045-2050. https://doi.org/10.1007/s007050050441

Recombination of baculovirus DNA following lipofection of insect larvae. / Hajós, J. P.; Zuidema, D.; Kulcsár, P.; Heldens, J. G M; Závodszky, P.; Vlak, J. M.

In: Archives of Virology, Vol. 143, No. 10, 1998, p. 2045-2050.

Research output: Contribution to journalArticle

Hajós, JP, Zuidema, D, Kulcsár, P, Heldens, JGM, Závodszky, P & Vlak, JM 1998, 'Recombination of baculovirus DNA following lipofection of insect larvae', Archives of Virology, vol. 143, no. 10, pp. 2045-2050. https://doi.org/10.1007/s007050050441
Hajós, J. P. ; Zuidema, D. ; Kulcsár, P. ; Heldens, J. G M ; Závodszky, P. ; Vlak, J. M. / Recombination of baculovirus DNA following lipofection of insect larvae. In: Archives of Virology. 1998 ; Vol. 143, No. 10. pp. 2045-2050.
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