Recombinant protein expression in milk of livestock species

Zsuzsanna Bösze, László Hiripi

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Producing complex recombinant proteins in the milk of transgenic animals offers several advantages: large amounts of proteins can be obtained, and in most cases, these proteins are properly folded, assembled, cleaved, and glycosylated. The level of expression of foreign genes in the mammalian gland cannot be predicted in all cases, and appropriate vectors must be used. The main elements of these vectors are as follows: A well-characterized specific promoter, the coding region of the gene of interest, preferably with a homologous or heterologous intron, to improve transcription efficiency, and an insulator or boundary element to counteract the chromosomal position effects at the integration site. Once high expression levels are achieved, and the recombinant protein is purified, an essential step in the analysis of the final product is determining its degree of glycosylation. This is an important readout because it can affect among other parameters the stability and immunogenicity of the recombinant protein.

Original languageEnglish
Title of host publicationRecombinant Gene Expression
Subtitle of host publicationReviews and Protocols, Third Edition
EditorsArgelia Lorence
Pages629-641
Number of pages13
DOIs
Publication statusPublished - Jan 23 2012

Publication series

NameMethods in Molecular Biology
Volume824
ISSN (Print)1064-3745

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Keywords

  • Expression vectors
  • Milk
  • Posttranslational modifications
  • Purification
  • Recombinant proteins
  • Transgenic animals

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bösze, Z., & Hiripi, L. (2012). Recombinant protein expression in milk of livestock species. In A. Lorence (Ed.), Recombinant Gene Expression: Reviews and Protocols, Third Edition (pp. 629-641). (Methods in Molecular Biology; Vol. 824). https://doi.org/10.1007/978-1-61779-433-9_34