A rekombináns humán luteinizáló hormon és choriogonadotrop hormon hatásai a humán granulózasejtek ösztradiol bioszintézisére: Az androgének szerepének vizsgálata a bazális és gonadotropin-függo ösztrogén termelésben

Translated title of the contribution: Recombinant gonadotropin-mediated granulosa cell estradiol production, evaluation of the direct effects of androgens on basal and stimulated steroid biosynthesis

I. Földesi, M. Breckwoldt, J. Neulen

Research output: Contribution to journalArticle

Abstract

The aim of the work was to investigate the effects of recombinant LH (r1.H) and recombinant HCG (rHCG) on oestradiol production of granulosa cells harvested for in vitro fertilization. In addition, the possible role of androgens in the basal and recombinant FSH (rFSH)-dependent estrogen biosynthesis was also studied. All experiments were performed after a preincubation period for 3 days to allow the cells to recover following hyperstimulation. Both in the absence and in the presence of added androgens, r1.H did not effect estradiol production. In contrast, differences were found in granulosa cell responses to rHCG with respect to oestradiol production. Recombinant HCG inhibited estrogen synthesis within days 3 and 5 of the culture, being significant at doses higher than 0,01 IU/ml, whereas no effect was found during the next treatment interval (between days 7 and 9). rHCG tended to increase the oestradiol levels, with a significant stimulation occurring at a dose of 0,1 IU/ml. In spite of poor responses to r1.H and rHCG stimulation, granulosa cells displayed high aromatase activity as demonstrated by the increases in estradiol production in the presence of testosterone. Furthermore, these cells were also sensitive to gonadotropin stimulation as evidenced by responses of granulosa cells to rFSH induction. However, 5 -dihydrotestosterone, a non-aromatizable androgen, had no influence on either the basal or rFSH-stimulated estradiol production. From our resuits the following conclusions could be drawn: (i) r1.H, applied in near physiological doses, had no effect on the estdiol production; (ii) rHCG tended to stimulate granulosa cell estrogen production after an initial refractory stage; (iii) under these culture conditions, androgens had no direct effect on basal and rFSH-induced estradiol production, their significance appears to be restricted to substrates necessary for estrogen biosynthesis.

Original languageHungarian
Pages (from-to)35-40
Number of pages6
JournalMagyar Noorvosok Lapja
Volume64
Issue number1
Publication statusPublished - Jan 1 2001

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ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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