Recirculated normal platelets adhere to surfaces coated with plasma from patients with immune thrombocytopenia

M. A. Azerad, J. Hársfalvi, H. Deckmyn, J. Vermylen, J. L. Michaux, M. F. Hoylaerts

Research output: Contribution to journalArticle

Abstract

Immune thrombocytopenic purpura (ITP) patients have characteristic anti-platelet antibodies in their circulation. To assess the interaction between such antibodies adhering on to a non-physiological surface and human platelets, normal anticoagulated blood was perfused over ITP patient plasma-coated surfaces in a parallel plate flow chamber. At 300 s-1, platelet adhesion to patient plasma-coated glass coverslips (24.0 ± 10%) was significantly higher than the adhesion to normal plasma-coated surfaces (9.8 ± 7%). When perfused at 1300 s-1, the adhesion to patient plasma- (5.1 ± 1.3%) and to normal plasma- (2.5 ± 1.2%) coated coverslips were significantly weaker. Furthermore, patient platelet binding depended on simultaneous contributions by antibodies and fibrinogen present on the plasma-coated surface, since adherence was antagonized both by normal immunoglobulins added to the perfusate, as well as by the anti-CPIIb/IIIa monoclonal antibody 16N7C2, which competes with fibrinogen for binding to its receptor on the platelet. Accordingly, platelet adhesion was only observed to coverslips coated with the plasma but not the serum of ITP patients. Hence, perfusion of normal platelets over surfaces coated with ITP patient plasma enables a functional assessment of the presence in this plasma of anti-platelet antibodies.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalBlood Coagulation and Fibrinolysis
Volume8
Issue number1
Publication statusPublished - 1997

Fingerprint

Idiopathic Thrombocytopenic Purpura
Blood Platelets
Fibrinogen
Anti-Idiotypic Antibodies
Antibodies
Glass
Immunoglobulins
Perfusion
Monoclonal Antibodies

Keywords

  • anti-platelet antibodies
  • glycoprotein IIb/IIIa
  • immune suppression
  • immune thrombocytopenia
  • shear-induced platelet adhesion

ASJC Scopus subject areas

  • Hematology

Cite this

Recirculated normal platelets adhere to surfaces coated with plasma from patients with immune thrombocytopenia. / Azerad, M. A.; Hársfalvi, J.; Deckmyn, H.; Vermylen, J.; Michaux, J. L.; Hoylaerts, M. F.

In: Blood Coagulation and Fibrinolysis, Vol. 8, No. 1, 1997, p. 59-64.

Research output: Contribution to journalArticle

Azerad, M. A. ; Hársfalvi, J. ; Deckmyn, H. ; Vermylen, J. ; Michaux, J. L. ; Hoylaerts, M. F. / Recirculated normal platelets adhere to surfaces coated with plasma from patients with immune thrombocytopenia. In: Blood Coagulation and Fibrinolysis. 1997 ; Vol. 8, No. 1. pp. 59-64.
@article{de373b9b57ca49398387190ee328c486,
title = "Recirculated normal platelets adhere to surfaces coated with plasma from patients with immune thrombocytopenia",
abstract = "Immune thrombocytopenic purpura (ITP) patients have characteristic anti-platelet antibodies in their circulation. To assess the interaction between such antibodies adhering on to a non-physiological surface and human platelets, normal anticoagulated blood was perfused over ITP patient plasma-coated surfaces in a parallel plate flow chamber. At 300 s-1, platelet adhesion to patient plasma-coated glass coverslips (24.0 ± 10{\%}) was significantly higher than the adhesion to normal plasma-coated surfaces (9.8 ± 7{\%}). When perfused at 1300 s-1, the adhesion to patient plasma- (5.1 ± 1.3{\%}) and to normal plasma- (2.5 ± 1.2{\%}) coated coverslips were significantly weaker. Furthermore, patient platelet binding depended on simultaneous contributions by antibodies and fibrinogen present on the plasma-coated surface, since adherence was antagonized both by normal immunoglobulins added to the perfusate, as well as by the anti-CPIIb/IIIa monoclonal antibody 16N7C2, which competes with fibrinogen for binding to its receptor on the platelet. Accordingly, platelet adhesion was only observed to coverslips coated with the plasma but not the serum of ITP patients. Hence, perfusion of normal platelets over surfaces coated with ITP patient plasma enables a functional assessment of the presence in this plasma of anti-platelet antibodies.",
keywords = "anti-platelet antibodies, glycoprotein IIb/IIIa, immune suppression, immune thrombocytopenia, shear-induced platelet adhesion",
author = "Azerad, {M. A.} and J. H{\'a}rsfalvi and H. Deckmyn and J. Vermylen and Michaux, {J. L.} and Hoylaerts, {M. F.}",
year = "1997",
language = "English",
volume = "8",
pages = "59--64",
journal = "Blood Coagulation and Fibrinolysis",
issn = "0957-5235",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Recirculated normal platelets adhere to surfaces coated with plasma from patients with immune thrombocytopenia

AU - Azerad, M. A.

AU - Hársfalvi, J.

AU - Deckmyn, H.

AU - Vermylen, J.

AU - Michaux, J. L.

AU - Hoylaerts, M. F.

PY - 1997

Y1 - 1997

N2 - Immune thrombocytopenic purpura (ITP) patients have characteristic anti-platelet antibodies in their circulation. To assess the interaction between such antibodies adhering on to a non-physiological surface and human platelets, normal anticoagulated blood was perfused over ITP patient plasma-coated surfaces in a parallel plate flow chamber. At 300 s-1, platelet adhesion to patient plasma-coated glass coverslips (24.0 ± 10%) was significantly higher than the adhesion to normal plasma-coated surfaces (9.8 ± 7%). When perfused at 1300 s-1, the adhesion to patient plasma- (5.1 ± 1.3%) and to normal plasma- (2.5 ± 1.2%) coated coverslips were significantly weaker. Furthermore, patient platelet binding depended on simultaneous contributions by antibodies and fibrinogen present on the plasma-coated surface, since adherence was antagonized both by normal immunoglobulins added to the perfusate, as well as by the anti-CPIIb/IIIa monoclonal antibody 16N7C2, which competes with fibrinogen for binding to its receptor on the platelet. Accordingly, platelet adhesion was only observed to coverslips coated with the plasma but not the serum of ITP patients. Hence, perfusion of normal platelets over surfaces coated with ITP patient plasma enables a functional assessment of the presence in this plasma of anti-platelet antibodies.

AB - Immune thrombocytopenic purpura (ITP) patients have characteristic anti-platelet antibodies in their circulation. To assess the interaction between such antibodies adhering on to a non-physiological surface and human platelets, normal anticoagulated blood was perfused over ITP patient plasma-coated surfaces in a parallel plate flow chamber. At 300 s-1, platelet adhesion to patient plasma-coated glass coverslips (24.0 ± 10%) was significantly higher than the adhesion to normal plasma-coated surfaces (9.8 ± 7%). When perfused at 1300 s-1, the adhesion to patient plasma- (5.1 ± 1.3%) and to normal plasma- (2.5 ± 1.2%) coated coverslips were significantly weaker. Furthermore, patient platelet binding depended on simultaneous contributions by antibodies and fibrinogen present on the plasma-coated surface, since adherence was antagonized both by normal immunoglobulins added to the perfusate, as well as by the anti-CPIIb/IIIa monoclonal antibody 16N7C2, which competes with fibrinogen for binding to its receptor on the platelet. Accordingly, platelet adhesion was only observed to coverslips coated with the plasma but not the serum of ITP patients. Hence, perfusion of normal platelets over surfaces coated with ITP patient plasma enables a functional assessment of the presence in this plasma of anti-platelet antibodies.

KW - anti-platelet antibodies

KW - glycoprotein IIb/IIIa

KW - immune suppression

KW - immune thrombocytopenia

KW - shear-induced platelet adhesion

UR - http://www.scopus.com/inward/record.url?scp=0031005771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031005771&partnerID=8YFLogxK

M3 - Article

C2 - 9105639

AN - SCOPUS:0031005771

VL - 8

SP - 59

EP - 64

JO - Blood Coagulation and Fibrinolysis

JF - Blood Coagulation and Fibrinolysis

SN - 0957-5235

IS - 1

ER -