Reciprocal enhancement of gene expression and viral replication between human cytomegalovirus and human immunodeficiency virus type 1

W. Z. Ho, J. M. Harouse, R. F. Rando, E. Gönczöl, A. Srinivasan, S. A. Plotkin

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Abstract

Biological interactions between human cytomegalovirus (HCMV) and the human immunodeficiency virus type 1 (HIV-1) were analysed in transfection and infection experiments, carried out in a human osteogenic sarcoma cell line (HOS) and in the same cell line chronically infected with HCMV (E155). When HOS and E155 cells were transfected with recombinant plasmids containing the HIV long terminal repeat (LTR) linked to the bacterial chloramphenicol acetyltransferase (CAT) gene, LTR-directed CAT expression was 20 times higher in E155 cells than in HOS cells. HOS cells co-infected with HCMV and HIV-1 showed enhanced production of the HIV-1 p24 antigen. In reciprocal experiments, an increase in HCMV immediate early gene expression was observed when HCMV-infected HOS cells and E155 cells were either transfected with a recombinant plasmid containing the HIV transactivator gene (pTAT), or when infected with HIV-1. DNA hybridization analysis of E155 and HCMV-infected HOS cells revealed higher levels of HCMV DNA in cells transfected with pTAT than in cells transfected with other non-specific recombinant plasmids. E155 cells transfected with pTAT also produced higher titres of infectious HCMV than control cultures of E155 cells transfected with other recombinant plasmids, including pMTAT carrying a mutant tat gene. The functional reciprocity in vitro between HCMV and HIV is discussed with respect to its possible implications for the clinical development of AIDS.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalJournal of General Virology
Volume71
Issue number1
Publication statusPublished - 1990

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Cytomegalovirus
HIV-1
Gene Expression
Plasmids
Chloramphenicol O-Acetyltransferase
tat Genes
HIV Long Terminal Repeat
HIV
Cell Line
Immediate-Early Genes
Trans-Activators
Terminal Repeat Sequences
DNA
Osteosarcoma
Genes
Transfection
Acquired Immunodeficiency Syndrome
Cell Culture Techniques
Antigens
Infection

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Reciprocal enhancement of gene expression and viral replication between human cytomegalovirus and human immunodeficiency virus type 1. / Ho, W. Z.; Harouse, J. M.; Rando, R. F.; Gönczöl, E.; Srinivasan, A.; Plotkin, S. A.

In: Journal of General Virology, Vol. 71, No. 1, 1990, p. 97-103.

Research output: Contribution to journalArticle

Ho, W. Z. ; Harouse, J. M. ; Rando, R. F. ; Gönczöl, E. ; Srinivasan, A. ; Plotkin, S. A. / Reciprocal enhancement of gene expression and viral replication between human cytomegalovirus and human immunodeficiency virus type 1. In: Journal of General Virology. 1990 ; Vol. 71, No. 1. pp. 97-103.
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