Recent progress in the elucidation of the function of noda, b, and c genes of rhizobium meuloti

Jürgen Schmidt, Michael John, É. Kondorosi, Adam Kondorosi, Jeff Schell

Research output: Contribution to journalArticle

Abstract

The common nodulation genes nodA, B, and C are highly conserved between different Rhizobium species and are required for the nodulation of legumes and non-legumes. The expression of these genes in Rhizobium meliloti is under both positive and negative control. Monospecific polyclonal antibodies were used to localize the NodA and NodB proteins in the cytosol of R. meliloti. These proteins are involved in the generation of small heat-stable compounds that stimulate the mitosis of different plant protoplasts. Our experiments suggest that the NodC transmembrane protein is not involved in the synthesis of these factors. Gene fusion experiments were used to define the membrane- anchor domain which is necessary for the insertion of the NodC protein into the membrane. A highly hydrophobic transmembrane-anchor domain was found near the carboxyl terminus, separating a large extracellular domain which contains a cystein-rich cluster from a short putative intracellular domain. The domain structure of the dimeric NodC protein shows striking similarities with cell-surface receptors of eukaryotes. A truncated form of NodC was detected in nodules of various legumes. The possible role of NodC in signal transduction from the bacterial cell to the plant cell is discussed.

Original languageEnglish
Pages (from-to)165-169
Number of pages5
JournalIsrael Journal of Botany
Volume40
Issue number2
DOIs
Publication statusPublished - Jan 1 1991

Fingerprint

rhizobacterium
Rhizobium
Ensifer meliloti
nodulation
protein
gene
legumes
transmembrane proteins
anchor
genes
gene fusion
proteins
cells
polyclonal antibodies
cytosol
membrane proteins
mitosis
protoplasts
eukaryotic cells
signal transduction

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Recent progress in the elucidation of the function of noda, b, and c genes of rhizobium meuloti. / Schmidt, Jürgen; John, Michael; Kondorosi, É.; Kondorosi, Adam; Schell, Jeff.

In: Israel Journal of Botany, Vol. 40, No. 2, 01.01.1991, p. 165-169.

Research output: Contribution to journalArticle

Schmidt, Jürgen ; John, Michael ; Kondorosi, É. ; Kondorosi, Adam ; Schell, Jeff. / Recent progress in the elucidation of the function of noda, b, and c genes of rhizobium meuloti. In: Israel Journal of Botany. 1991 ; Vol. 40, No. 2. pp. 165-169.
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