Recent advances using FcRn overexpression in transgenic animals to overcome impediments of standard antibody technologies to improve the generation of specific antibodies

I. Kacskovics, Judit Cervenak, A. Erdei, Richard A. Goldsby, John E. Butler

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This review illustrates the salutary effects of neonatal Fc receptor (FcRn) overexpression in significantly improving humoral immune responses in the generation of antibodies for immunotherapy and diagnostics. These include: (1) improved IgG protection; (2) augmented antigen-specific humoral immune response with larger numbers of antigen specific B cells, thus offering a wider spectrum of clones; (3) generation of antibodies against weakly immunogenic antigens; (4) significant improvements in the number and substantial developments in the diversity of hybridomas. FcRn transgenesis thus confers a number of practical benefits, including faster antibody production, higher antibody yields and improved generation of hybridomas for monoclonal antibody production. Notably, these efficiencies in polyclonal antibody production were also demonstrated in FcRn transgenic rabbits. Overall, FcRn transgenic animals yield more antibodies and provide a route to the generation of antibodies against antigens of low immunogenicity that are difficult to obtain using currently available methods.

Original languageEnglish
Pages (from-to)431-439
Number of pages9
JournalmAbs
Volume3
Issue number5
DOIs
Publication statusPublished - Sep 2011

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Genetically Modified Animals
Technology
Antibodies
Antibody Formation
Antigens
Hybridomas
Humoral Immunity
Gene Transfer Techniques
Immunotherapy
B-Lymphocytes
Clone Cells
Immunoglobulin G
Monoclonal Antibodies
Rabbits

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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Recent advances using FcRn overexpression in transgenic animals to overcome impediments of standard antibody technologies to improve the generation of specific antibodies. / Kacskovics, I.; Cervenak, Judit; Erdei, A.; Goldsby, Richard A.; Butler, John E.

In: mAbs, Vol. 3, No. 5, 09.2011, p. 431-439.

Research output: Contribution to journalArticle

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