Real-time pure shift measurements for uniformly isotope-labeled molecules using X-selective BIRD homonuclear decoupling

Jens D. Haller, A. Bodor, Burkhard Luy

Research output: Contribution to journalArticle

Abstract

We introduce a novel selective inversion element for chunked homonuclear decoupling that combines isotope selection via BIRD-filtering with band-selective inversion on the X-heteronucleus and allows efficient real-time decoupling of homonuclear and heteronuclear couplings. It is especially suitable for uniformly isotope-labeled compounds. We discuss in detail the inversion element based on band-selective refocusing on the X-nuclei (BASEREX), highlighting in particular the role of appropriate band-selective shaped refocusing pulses and the application of broadband X-pulses for an effective BIRD d element during homodecoupling. The approach is experimentally verified and studied in detail using uniformly 13 C-labeled glucose and a uniformly 15 N, 13 C-labeled amino acid mixture.

Original languageEnglish
Pages (from-to)64-71
Number of pages8
JournalJournal of Magnetic Resonance
Volume302
DOIs
Publication statusPublished - May 1 2019

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Keywords

  • Band selective
  • BASEREX
  • BIRD
  • Homonuclear decoupling
  • Inversion element
  • Isotope-labeling
  • Pure shift
  • Real-time

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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