Real-time PCR

Revolutionizing detection and expression analysis of genes

S. A. Deepak, K. R. Kottapalli, R. Rakwal, G. Oros, K. S. Rangappa, H. Iwahashi, Y. Masuo, G. K. Agrawal

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis of gene(s) in real-time - has revolutionized the 21st century biological science due to its tremendous application in quantitative genotyping, genetic variation of inter and intra organisms, early diagnosis of disease, forensic, to name a few. We comprehensively review various aspects of real-time PCR, including technological refinement and application in all scientific fields ranging from medical to environmental issues, and to plant.

Original languageEnglish
Pages (from-to)234-251
Number of pages18
JournalCurrent Genomics
Volume8
Issue number4
DOIs
Publication statusPublished - Aug 2007

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Real-Time Polymerase Chain Reaction
Gene Expression
Biological Science Disciplines
Names
Early Diagnosis
Parturition
Technology
Polymerase Chain Reaction

Keywords

  • Applications
  • Detection
  • Disease
  • Microorganisms
  • Pathogen
  • Plant-microbe interaction
  • Quantification
  • Real-time PCR

ASJC Scopus subject areas

  • Genetics

Cite this

Deepak, S. A., Kottapalli, K. R., Rakwal, R., Oros, G., Rangappa, K. S., Iwahashi, H., ... Agrawal, G. K. (2007). Real-time PCR: Revolutionizing detection and expression analysis of genes. Current Genomics, 8(4), 234-251. https://doi.org/10.2174/138920207781386960

Real-time PCR : Revolutionizing detection and expression analysis of genes. / Deepak, S. A.; Kottapalli, K. R.; Rakwal, R.; Oros, G.; Rangappa, K. S.; Iwahashi, H.; Masuo, Y.; Agrawal, G. K.

In: Current Genomics, Vol. 8, No. 4, 08.2007, p. 234-251.

Research output: Contribution to journalArticle

Deepak, SA, Kottapalli, KR, Rakwal, R, Oros, G, Rangappa, KS, Iwahashi, H, Masuo, Y & Agrawal, GK 2007, 'Real-time PCR: Revolutionizing detection and expression analysis of genes', Current Genomics, vol. 8, no. 4, pp. 234-251. https://doi.org/10.2174/138920207781386960
Deepak SA, Kottapalli KR, Rakwal R, Oros G, Rangappa KS, Iwahashi H et al. Real-time PCR: Revolutionizing detection and expression analysis of genes. Current Genomics. 2007 Aug;8(4):234-251. https://doi.org/10.2174/138920207781386960
Deepak, S. A. ; Kottapalli, K. R. ; Rakwal, R. ; Oros, G. ; Rangappa, K. S. ; Iwahashi, H. ; Masuo, Y. ; Agrawal, G. K. / Real-time PCR : Revolutionizing detection and expression analysis of genes. In: Current Genomics. 2007 ; Vol. 8, No. 4. pp. 234-251.
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