Real-time PCR: Revolutionizing detection and expression analysis of genes

S. A. Deepak, K. R. Kottapalli, R. Rakwal, G. Oros, K. S. Rangappa, H. Iwahashi, Y. Masuo, G. K. Agrawal

Research output: Contribution to journalReview article

80 Citations (Scopus)

Abstract

Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis of gene(s) in real-time - has revolutionized the 21st century biological science due to its tremendous application in quantitative genotyping, genetic variation of inter and intra organisms, early diagnosis of disease, forensic, to name a few. We comprehensively review various aspects of real-time PCR, including technological refinement and application in all scientific fields ranging from medical to environmental issues, and to plant.

Original languageEnglish
Pages (from-to)234-251
Number of pages18
JournalCurrent Genomics
Volume8
Issue number4
DOIs
Publication statusPublished - Aug 1 2007

Keywords

  • Applications
  • Detection
  • Disease
  • Microorganisms
  • Pathogen
  • Plant-microbe interaction
  • Quantification
  • Real-time PCR

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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  • Cite this

    Deepak, S. A., Kottapalli, K. R., Rakwal, R., Oros, G., Rangappa, K. S., Iwahashi, H., Masuo, Y., & Agrawal, G. K. (2007). Real-time PCR: Revolutionizing detection and expression analysis of genes. Current Genomics, 8(4), 234-251. https://doi.org/10.2174/138920207781386960