Real ribozymes suggest a relaxed error threshold

Ádám Kun, Mauro Santos, E. Szathmáry

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The error threshold for replication, the critical copying fidelity below which the fittest genotype deterministically disappears, limits the length of the genome that can be maintained by selection. Primordial replication must have been error-prone, and so early replicators are thought to have been necessarily short1. The error threshold also depends on the fitness landscape. In an RNA world2, many neutral and compensatory mutations can raise the threshold, below which the functional phenotype3, rather than a particular sequence, is still present4,5. Here we show, on the basis of comparative analysis of two extensively mutagenized ribozymes, that with a copying fidelity of 0.999 per digit per replication the phenotypic error threshold rises well above 7,000 nucleotides, which permits the selective maintenance of a functionally rich riboorganism6 with a genome of more than 100 different genes, the size of a tRNA. This requires an order of magnitude of improvement in the accuracy of in vitro-generated polymerase ribozymes7,8. Incidentally, this genome size coincides with that estimated for a minimal cell achieved by top-down analysis9, omitting the genes dealing with translation.

Original languageEnglish
Pages (from-to)1008-1011
Number of pages4
JournalNature Genetics
Volume37
Issue number9
DOIs
Publication statusPublished - Sep 2005

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Catalytic RNA
Genome
Genome Size
Transfer RNA
Genes
Nucleotides
Genotype
Maintenance
RNA
Mutation
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Real ribozymes suggest a relaxed error threshold. / Kun, Ádám; Santos, Mauro; Szathmáry, E.

In: Nature Genetics, Vol. 37, No. 9, 09.2005, p. 1008-1011.

Research output: Contribution to journalArticle

Kun, Ádám ; Santos, Mauro ; Szathmáry, E. / Real ribozymes suggest a relaxed error threshold. In: Nature Genetics. 2005 ; Vol. 37, No. 9. pp. 1008-1011.
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