Reactivity of lymphocytes to a progesterone receptor-specific monoclonal antibody

Julia Szekeres-Bartho, Gy Szekeres, P. Debre, B. Autran, G. Chaouat

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

In this study we present evidence for reactivity of pregnancy lymphocytes, but not nonpregnancy lymphocytes, with the progesterone receptor-specific monoclonal antibody mPRI. Using an avidin-biotin peroxidase detection system, we found a nuclear staining in 14.6 ± 3.7% (mean ± SEM, N = 27) of pregnancy lymphocytes, while only 0.47 ± 0.33% (mean ± SEM, N = 15) of nonpregnancy lymphocytes reacted with the antibody. To characterize the receptor-bearing subset, CD8+ and CD4+ cells were depleted by complement-dependent lysis. Depletion of CD8+ cells was accompanied by 62 ± 18% loss of progesterone receptor-bearing cells, while depletion of CD4+ cells resulted in a twofold increase in the number of positively staining lymphocytes. In nonpregnancy lymphocytes a 3-day PHA treatment, as well as allogeneic stimulation, resulted in a significant increase in the number of receptor-containing cells. These results suggest that pregnancy, but not nonpregnancy, lymphocytes contain progesterone binding structures, and that these are inducible by mitogenic or alloantigenic stimuli.

Original languageEnglish
Pages (from-to)273-283
Number of pages11
JournalCellular Immunology
Volume125
Issue number2
DOIs
Publication statusPublished - Feb 1990

ASJC Scopus subject areas

  • Immunology

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