Reactive glia support and guide axon growth in the rat thalamus during the first postnatal week. A sharply timed transition from permissive to non-permissive stage

Béla M. Ajtai, M. Kálmán

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The present study demonstrates a supportive and guiding effect of the reactive glia on the postlesional axon growth in vivo, and offers a model system to compare permissive and non-permissive forms of the glial reaction. After stab wounds in early postnatal (P2-P9) rats, the reactive glia and the nerve fibers were detected by the immunohistochemical staining of glial fibrillary acidic protein (GFAP) and neurofilament protein, respectively. In the thalamus of the animals lesioned at P5 or earlier, an extraordinary bundle of fibers immunoreactive to neurofilament protein was found, corresponding to the lesion track marked by reactive glia. This bundle persisted up to 2 months, as shown by electron microscopy. When the animals were lesioned at P7 or later, the lesion track was immunonegative to neurofilament protein. Following P6 lesions, an intermediate situation was found, the strip of immunoreactive neurofilament protein was missing, or short and weak. GFAP immunostaining demonstrated a typical reactive glia in every case. As a result of the same operation, reactive glia plus a deficiency of neurofilament protein immunostaining was found in every animal in the cortex and the corpus callosum, independently from the age at lesion. The results demonstrate that the permissive nature of the glial reaction depends on the lesioned area as well, and changes to a non-permissive effect in a short time interval.

Original languageEnglish
Pages (from-to)589-597
Number of pages9
JournalInternational Journal of Developmental Neuroscience
Volume19
Issue number6
DOIs
Publication statusPublished - 2001

Fingerprint

Thalamus
Neuroglia
Neurofilament Proteins
Axons
Growth
Glial Fibrillary Acidic Protein
Stab Wounds
Corpus Callosum
Nerve Fibers
Electron Microscopy
Staining and Labeling

Keywords

  • Astroglia
  • Glial fibrillary acidic protein
  • Neural regeneration
  • Neurofilament protein
  • Reactive glia

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

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title = "Reactive glia support and guide axon growth in the rat thalamus during the first postnatal week. A sharply timed transition from permissive to non-permissive stage",
abstract = "The present study demonstrates a supportive and guiding effect of the reactive glia on the postlesional axon growth in vivo, and offers a model system to compare permissive and non-permissive forms of the glial reaction. After stab wounds in early postnatal (P2-P9) rats, the reactive glia and the nerve fibers were detected by the immunohistochemical staining of glial fibrillary acidic protein (GFAP) and neurofilament protein, respectively. In the thalamus of the animals lesioned at P5 or earlier, an extraordinary bundle of fibers immunoreactive to neurofilament protein was found, corresponding to the lesion track marked by reactive glia. This bundle persisted up to 2 months, as shown by electron microscopy. When the animals were lesioned at P7 or later, the lesion track was immunonegative to neurofilament protein. Following P6 lesions, an intermediate situation was found, the strip of immunoreactive neurofilament protein was missing, or short and weak. GFAP immunostaining demonstrated a typical reactive glia in every case. As a result of the same operation, reactive glia plus a deficiency of neurofilament protein immunostaining was found in every animal in the cortex and the corpus callosum, independently from the age at lesion. The results demonstrate that the permissive nature of the glial reaction depends on the lesioned area as well, and changes to a non-permissive effect in a short time interval.",
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AU - Ajtai, Béla M.

AU - Kálmán, M.

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N2 - The present study demonstrates a supportive and guiding effect of the reactive glia on the postlesional axon growth in vivo, and offers a model system to compare permissive and non-permissive forms of the glial reaction. After stab wounds in early postnatal (P2-P9) rats, the reactive glia and the nerve fibers were detected by the immunohistochemical staining of glial fibrillary acidic protein (GFAP) and neurofilament protein, respectively. In the thalamus of the animals lesioned at P5 or earlier, an extraordinary bundle of fibers immunoreactive to neurofilament protein was found, corresponding to the lesion track marked by reactive glia. This bundle persisted up to 2 months, as shown by electron microscopy. When the animals were lesioned at P7 or later, the lesion track was immunonegative to neurofilament protein. Following P6 lesions, an intermediate situation was found, the strip of immunoreactive neurofilament protein was missing, or short and weak. GFAP immunostaining demonstrated a typical reactive glia in every case. As a result of the same operation, reactive glia plus a deficiency of neurofilament protein immunostaining was found in every animal in the cortex and the corpus callosum, independently from the age at lesion. The results demonstrate that the permissive nature of the glial reaction depends on the lesioned area as well, and changes to a non-permissive effect in a short time interval.

AB - The present study demonstrates a supportive and guiding effect of the reactive glia on the postlesional axon growth in vivo, and offers a model system to compare permissive and non-permissive forms of the glial reaction. After stab wounds in early postnatal (P2-P9) rats, the reactive glia and the nerve fibers were detected by the immunohistochemical staining of glial fibrillary acidic protein (GFAP) and neurofilament protein, respectively. In the thalamus of the animals lesioned at P5 or earlier, an extraordinary bundle of fibers immunoreactive to neurofilament protein was found, corresponding to the lesion track marked by reactive glia. This bundle persisted up to 2 months, as shown by electron microscopy. When the animals were lesioned at P7 or later, the lesion track was immunonegative to neurofilament protein. Following P6 lesions, an intermediate situation was found, the strip of immunoreactive neurofilament protein was missing, or short and weak. GFAP immunostaining demonstrated a typical reactive glia in every case. As a result of the same operation, reactive glia plus a deficiency of neurofilament protein immunostaining was found in every animal in the cortex and the corpus callosum, independently from the age at lesion. The results demonstrate that the permissive nature of the glial reaction depends on the lesioned area as well, and changes to a non-permissive effect in a short time interval.

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