Reactions of tobacco genotypes with different antioxidant capacities to powdery mildew and Tobacco mosaic virus infections

G. Gullner, Csilla Juhász, Adél Németh, B. Barna

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The interactions of powdery mildew (Golovinomyces orontii) and Tobacco mosaic virus (TMV) with tobacco lines having down or upregulated antioxidants were investigated. Xanthi-nc, its salicylic acid-deficient NahG mutant, a paraquat-sensitive Samsun (PS) and its paraquat tolerant (PT) mutant were used. Cell membrane damage caused by H2O2 was significantly higher in NahG than Xanthi, whereas it was lower in PT than in PS. Leakage of ions from PT was reduced by the powdery mildew infection. On the other hand TMV inoculation led to a 6-fold and 2-fold elevation of ion leakage from hypersensitive resistant NahG and Xanthi leaves, respectively, whereas ion leakage increased slightly from susceptible PS leaves. G. orontii infection induced ribonuclease (RNase) enzyme activity in extracts from Xanthi and NahG (about 200–250% increase) and weakly (about 20–30% increase) from PS and PT lines. Pre-treatment with protein kinase inhibitor staurosporine or protein phosphatase inhibitor okadaic acid very strongly inhibited mildew development on tobacco lines. Our experiments suggest that protein kinases inhibited by staurosporine seem to be important factors, while protein phosphatases inhibited by okadaic acid play less significant role in TMV-induced lesion development. Both powdery mildew and TMV infections up-regulated the expression of PR-1b, PR-1c and WRKY12 genes in all tobacco lines to various extents.

Original languageEnglish
Pages (from-to)232-239
Number of pages8
JournalPlant Physiology and Biochemistry
Volume119
DOIs
Publication statusPublished - Oct 1 2017

    Fingerprint

Keywords

  • Ion leakage
  • NahG tobacco
  • Okadaic acid
  • PAGE RNase pattern
  • Paraquat tolerant tobacco
  • PR1 gene
  • Staurosporine

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this