Reaction of glutaraldehyde with NH2 compounds. A spectrophotometric method for the determination of glutaraldehyde concentration

János Hajdu, Peter Friedrich

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Glutaraldehyde reacts with an excess of hydroxylamine, hydrazine or methylamine through an intermediate that absorbs more strongly at 240 nm than do the end products. On the basis of the characteristic absorbance change during the reaction with hydroxylamine a spectrophotometric difference method was developed for the determination of glutaraldehyde concentration which is not disturbed by the presence of other materials absorbing in the ultraviolet.

Original languageEnglish
Pages (from-to)273-280
Number of pages8
JournalAnalytical Biochemistry
Volume65
Issue number1-2
DOIs
Publication statusPublished - May 12 1975

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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