Rat substrains differ in the magnitude of spontaneous locomotor recovery and in the development of mechanical hypersensitivity after experimental spinal cord injury

Jacob Kjell, K. Sándor, Anna Josephson, Camilla I. Svensson, Mathew B. Abrams

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A number of different rodent experimental models of spinal cord injury have been used in an attempt to model the pathophysiology of human spinal cord injury. As a result, interlaboratory comparisons of the outcome measures can be difficult. Further complicating interexperiment comparisons is the fact that the rodent response to different experimental models is strain-dependent. Moreover, the literature is abundant with examples in which the same injury model and strain result in divergent functional outcomes. The objective of this research was to determine whether substrain differences influence functional outcome in experimental spinal cord injury. We induced mild contusion spinal cord injuries in three substrains of Sprague-Dawley rats purchased from three different European breeders (Scanbur, Charles River, and Harlan) and evaluated the impact of injury on spontaneous locomotor function, hypersensitivity to mechanical stimulation, and bladder function. We found that Harlan rats regained significantly more hindlimb function than Charles River and Scanbur rats. We also observed substrain differences in the recovery of the ability to empty the bladder and development of hypersensitivity to mechanical stimulation. The Harlan substrain did not show any signs of hypersensitivity in contrast to the Scanbur and Charles River substrains, which both showed transient reduction in paw withdrawal thresholds. Lastly, we found histological differences possibly explaining the observed behavioral differences. We conclude that in spite of being the same strain, there might be genetic differences that can influence outcome measures in experimental studies of spinal cord injury of Sprague-Dawley rats from different vendors.

Original languageEnglish
Pages (from-to)1805-1811
Number of pages7
JournalJournal of Neurotrauma
Volume30
Issue number21
DOIs
Publication statusPublished - Nov 1 2013

Fingerprint

Spinal Cord Injuries
Hypersensitivity
Rivers
Sprague Dawley Rats
Rodentia
Urinary Bladder
Theoretical Models
Outcome Assessment (Health Care)
Contusions
Wounds and Injuries
Hindlimb
Research

Keywords

  • locomotor function
  • sensory function
  • spinal cord injury
  • strain; substrain

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Rat substrains differ in the magnitude of spontaneous locomotor recovery and in the development of mechanical hypersensitivity after experimental spinal cord injury. / Kjell, Jacob; Sándor, K.; Josephson, Anna; Svensson, Camilla I.; Abrams, Mathew B.

In: Journal of Neurotrauma, Vol. 30, No. 21, 01.11.2013, p. 1805-1811.

Research output: Contribution to journalArticle

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