Ras-dependence of nerve growth factor-induced inhibition of proliferation of PC12 cells

Katalin Kiss, B. Bartek, Nóra Nusser, J. Szeberenyi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The PC12 rat phaeochromocytoma cell line is the most frequently used model system to study neuronal differentiation: nerve growth factor (NGF) inhibits proliferation and causes easily scorable neurite formation in these cells. The central role of the monomeric guanine nucleotide binding Ras proteins in the pathway of NGF-induced differentiation is well documented. However, the possible involvement of Ras proteins in signal transduction processes mediating NGF-induced inhibition of proliferation has not been analyzed. To address this problem we studied PC12 subclones expressing a dominant inhibitory mutant Ras protein (Ha-Ras Asn17). Our results indicate that the cell cycle arrest characteristic of NGF-treated PC12 cells is mediated by a Ras-dependent signaling mechanism.

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalActa biologica Hungarica
Volume49
Issue number1
Publication statusPublished - Dec 1 1998

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Keywords

  • Ha-Ras Asn17 mutant
  • Nerve growth factor
  • PC12 cells
  • Proliferation
  • Ras proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

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