Rapid separation of rat peritoneal mast cells with Percoll

A. Nemeth, P. Rohlich

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Abstract

Rat peritoneal mast cells were separated by using density gradients of PVP-coated silica particles (Percoll). Mast cells were either isolated on preformed Percoll gradients or cell separation was made simultaneously with the gradient formation. Both procedures resulted mutaneously with the gradient formation. Both procedures resulted in mast cell suspensions of 95 to 99% purity. As tested by Ruthenium red staining and electron microscopy, the isolated mast cells showed a very good preservation of cell structure and reacted easily to the degranulating agent Compound 48/80. Practically all mast cells could be recovered from the peritoneal cell suspension. Percoll was found to be superior to earlier isolation procedures by giving a practically pure and intact mast cell suspension and by avoiding cell aggregation.

Original languageEnglish
Pages (from-to)272-275
Number of pages4
JournalEuropean Journal of Cell Biology
Volume20
Issue number3
Publication statusPublished - Jan 1 1980

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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