Rapid screening of natural products by high-resolution high-temperature gas chromatography

T. Cserháti, E. Forgács, Manuel Candeias, Louis Vilas-Boas, Rosario Bronze, Isabell Spranger

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The crude ethanol extracts from the leaves of three Croton hemiargyrus hemiargyreus plants are fractionated by thin-layer chromatography, yielding five fractions. The fractions and the crude extract are analyzed by high-temperature high-resolution gas chromatography coupled with mass spectrometry (HT-HRGC-MS). Several natural products, including thermolabile components, can be characterized directly in the samples, such as alkaloids, terpenes, flavonoids, acids, alcohols, etc. The cold on-column technique proves to be appropriate for the injection of these thermolabile compounds. HT-HRGC-MS is shown to be a valuable tool and an alternative technique to classical phytochemical procedures for the simple and fast routine analysis of natural products in crude extracts.

Original languageEnglish
Pages (from-to)234-240
Number of pages7
JournalJournal of Chromatographic Science
Volume38
Issue number6
Publication statusPublished - Jun 2000

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Biological Products
Complex Mixtures
Gas chromatography
Gas Chromatography
Mass spectrometry
Screening
Thin layer chromatography
Temperature
Mass Spectrometry
Terpenes
Phytochemicals
Croton
Alkaloids
Flavonoids
Ethanol
Alcohols
Thin Layer Chromatography
Acids
Injections

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Rapid screening of natural products by high-resolution high-temperature gas chromatography. / Cserháti, T.; Forgács, E.; Candeias, Manuel; Vilas-Boas, Louis; Bronze, Rosario; Spranger, Isabell.

In: Journal of Chromatographic Science, Vol. 38, No. 6, 06.2000, p. 234-240.

Research output: Contribution to journalArticle

Cserháti, T. ; Forgács, E. ; Candeias, Manuel ; Vilas-Boas, Louis ; Bronze, Rosario ; Spranger, Isabell. / Rapid screening of natural products by high-resolution high-temperature gas chromatography. In: Journal of Chromatographic Science. 2000 ; Vol. 38, No. 6. pp. 234-240.
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