Rapid screening method for arsenic speciation by combining thin layer chromatography and laser ablation-inductively coupled plasma-dynamic reaction cell-mass spectrometry

M. Resano, E. García Ruiz, V. Mihucz, A. Móricz, G. Záray, F. Vanhaecke

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Abstract

In this work, a rapid screening method for arsenic speciation, based on the use of polyethyleneimine (PEI) cellulose thin layer chromatographic (TLC) plates for separation and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) for the detection of the As species, has been implemented. The TLC plates were developed with a mixture consisting of acetone: acetic acid: water (2: 1: 1 v/v/v). The procedure proposed has been shown to be capable of the separation of picograms (an LOD of 3 pg has been calculated) to nanograms levels of arsenite [As(iii)], arsenate [As(v)], monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA), while arsenobetaine (AsB) and arsenocholine (AsC) practically remained on the sample application point of the TLC plate, allowing their separation from the rest of the compounds but not enabling separating them from one another. The separation procedure only requires approximately 20 min (up to 8 samples can be simultaneously separated) while the LA-ICP-MS protocol only needs a couple of minutes per chromatographic lane. The use of the dynamic reaction cell pressurized with H2 permits one to overcome the possible overlap of the signals of 75As+ and 40Ar35Cl+, ensuring reliable monitoring of the analyte species. The different As species found in NIST SRM 1568a rice flour, after prior enzymatically-assisted extraction with an ultrasonic processor, could be satisfactorily detected, suggesting that the method may be of interest for the fast and qualitative monitoring of As species in biological samples.

Original languageEnglish
Pages (from-to)1158-1162
Number of pages5
JournalJournal of Analytical Atomic Spectrometry
Volume22
Issue number9
DOIs
Publication statusPublished - 2007

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Thin layer chromatography
Arsenic
Inductively coupled plasma
Laser ablation
Mass spectrometry
Screening
Inductively coupled plasma mass spectrometry
Cacodylic Acid
Monitoring
Acetone
Acetic Acid
Ultrasonics
Water

ASJC Scopus subject areas

  • Spectroscopy

Cite this

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abstract = "In this work, a rapid screening method for arsenic speciation, based on the use of polyethyleneimine (PEI) cellulose thin layer chromatographic (TLC) plates for separation and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) for the detection of the As species, has been implemented. The TLC plates were developed with a mixture consisting of acetone: acetic acid: water (2: 1: 1 v/v/v). The procedure proposed has been shown to be capable of the separation of picograms (an LOD of 3 pg has been calculated) to nanograms levels of arsenite [As(iii)], arsenate [As(v)], monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA), while arsenobetaine (AsB) and arsenocholine (AsC) practically remained on the sample application point of the TLC plate, allowing their separation from the rest of the compounds but not enabling separating them from one another. The separation procedure only requires approximately 20 min (up to 8 samples can be simultaneously separated) while the LA-ICP-MS protocol only needs a couple of minutes per chromatographic lane. The use of the dynamic reaction cell pressurized with H2 permits one to overcome the possible overlap of the signals of 75As+ and 40Ar35Cl+, ensuring reliable monitoring of the analyte species. The different As species found in NIST SRM 1568a rice flour, after prior enzymatically-assisted extraction with an ultrasonic processor, could be satisfactorily detected, suggesting that the method may be of interest for the fast and qualitative monitoring of As species in biological samples.",
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T1 - Rapid screening method for arsenic speciation by combining thin layer chromatography and laser ablation-inductively coupled plasma-dynamic reaction cell-mass spectrometry

AU - Resano, M.

AU - García Ruiz, E.

AU - Mihucz, V.

AU - Móricz, A.

AU - Záray, G.

AU - Vanhaecke, F.

PY - 2007

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N2 - In this work, a rapid screening method for arsenic speciation, based on the use of polyethyleneimine (PEI) cellulose thin layer chromatographic (TLC) plates for separation and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) for the detection of the As species, has been implemented. The TLC plates were developed with a mixture consisting of acetone: acetic acid: water (2: 1: 1 v/v/v). The procedure proposed has been shown to be capable of the separation of picograms (an LOD of 3 pg has been calculated) to nanograms levels of arsenite [As(iii)], arsenate [As(v)], monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA), while arsenobetaine (AsB) and arsenocholine (AsC) practically remained on the sample application point of the TLC plate, allowing their separation from the rest of the compounds but not enabling separating them from one another. The separation procedure only requires approximately 20 min (up to 8 samples can be simultaneously separated) while the LA-ICP-MS protocol only needs a couple of minutes per chromatographic lane. The use of the dynamic reaction cell pressurized with H2 permits one to overcome the possible overlap of the signals of 75As+ and 40Ar35Cl+, ensuring reliable monitoring of the analyte species. The different As species found in NIST SRM 1568a rice flour, after prior enzymatically-assisted extraction with an ultrasonic processor, could be satisfactorily detected, suggesting that the method may be of interest for the fast and qualitative monitoring of As species in biological samples.

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